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Product Datasheet: ARP32557_P050 - GFI1 antibody - N-terminal region (ARP32557_P050) - Aviva Systems Biology
 
GFI1 antibody - N-terminal region (ARP32557_P050)
Data Sheet
 
Product Number ARP32557_P050
Product Page www.avivasysbio.com/gfi1-antibody-n-terminal-region-arp32557-p050.html
Product Name GFI1 antibody - N-terminal region (ARP32557_P050)
Size 100 ul
Gene Symbol GFI1
Alias Symbols ZNF163, SCN2, GFI-1
Protein Size (# AA) 422 amino acids
Molecular Weight 45kDa
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
NCBI Gene Id 2672
Host Rabbit
Clonality Polyclonal
Concentration Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
Official Gene Full Name Growth factor independent 1 transcription repressor
Description This is a rabbit polyclonal antibody against GFI1. It was validated on Western Blot using a cell lysate as a positive control. Aviva Systems Biology strives to provide antibodies covering each member of a whole protein family of your interest. We also use our best efforts to provide you antibodies recognize various epitopes of a target protein. For availability of antibody needed for your experiment, please inquire (info@avivasysbio.com).
Peptide Sequence Synthetic peptide located within the following region: VPAPSRADSTSNAGGAKAEPRDRLSPESQLTEAPDRASASPDSCEGSVCE
Target Reference Doan,L.L., et al., (2004) Nucleic Acids Res. 32 (8), 2508-2519
Description of Target GFI1 may be a transcription factor involved in regulating the expression of genes active in the S phase during cell cycle progression in T-cells. GFI1 may be involved in tumor progression. Defects in GFI1 are a cause of autosomal dominant severe congenital neutropenia (SCN) and dominant nonimmune chronic idiopathic neutropenia of adults (NI-CINA) This gene encodes a nuclear zinc finger protein that functions as a transcriptional repressor. This protein plays a role in diverse developmental contexts, including hematopoiesis and oncogenesis. It functions as part of a complex along with other cofactors to control histone modifications that lead to silencing of the target gene promoters. Mutations in this gene cause autosomal dominant severe congenital neutropenia, and also dominant nonimmune chronic idiopathic neutropenia of adults, which are heterogeneous hematopoietic disorders that cause predispositions to leukemias and infections. Multiple alternatively spliced variants, encoding the same protein, have been identified for this gene.
Protein Interactions RELA; SPI1; CHAF1A; PICK1; PIAS2; RCOR1; KDM1A; HDAC2; PRDM5; HDAC1; EHMT2; PIAS3; RUNX1T1; ARIH2; UBC;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Lead Time Domestic: within 1-2 days delivery International: 1-2 days
Blocking Peptide For anti-GFI1 (ARP32557_P050) antibody is Catalog # AAP32557 (Previous Catalog # AAPP32106)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human GFI1
Complete computational species homology data Anti-GFI1 (ARP32557_P050)
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express GFI1.
Swissprot Id Q99684
Protein Name Zinc finger protein Gfi-1
Protein Accession # NP_005254
Purification Affinity Purified
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express GFI1.
Nucleotide Accession # NM_005263
Replacement Item This antibody may replace item sc-101053 from Santa Cruz Biotechnology.
Conjugation Options

ARP32557_P050-FITC Conjugated

ARP32557_P050-HRP Conjugated

ARP32557_P050-Biotin Conjugated

CB Replacement sc-101053; sc-159136; sc-22796; sc-373960; sc-376949; sc-6357; sc-8558
Species Reactivity Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat
Application WB
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 93%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 93%; Pig: 100%; Rabbit: 79%; Rat: 100%
Image 1
Human Lung
WB Suggested Anti-GFI1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: Human Lung
 

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