WWTR1 Antibody - N-terminal region (ARP32540_P050)

• Product Number: ARP32540_P050
• Name: WWTR1 Antibody - N-terminal region (ARP32540_P050)
• Protein Size (# AA): 400 amino acids
• Molecular Weight: 44 kDa
• NCBI Gene Id: 25937
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: WW domain containing transcription regulator 1
• Alias Symbols: TAZ
• Peptide Sequence: Synthetic peptide located within the following region: PRLAGGAQHVRSHSSPASLQLGTGAGAAGSPAQQHAHLRQQSYDVTDELP
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Hong, J., et al., (2005) Science, 309(5737),1074-1078
• Description of Target: TAZ (transctiptional coactivator with PDZ-binding motif), also known as WW domain containing transcription regulator 1 (WWTR1), is a member of the bicoid sub-family of homeodomain-containing transcription factors. TAZ is a 14-3-3 binding protein that co-activates Runx2-dependent gene transcription while repressing PPAR-gamma-dependent gene transcription. TAZ has been reported to be a modulator of mesenchymal stem cell differentiation. TAZ may also play a role in brain and sensory organ development. A similar protein in mice is required for proper forebrain development. Two transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants may exist, but their full length sequences have not been determined.
• Protein Interactions: MED28; NOTCH1; YWHAQ; LATS1; BTRC; YWHAE; TP53BP2; PPP1CA; KLF5; YWHAZ; YWHAG; PAX3; SLC9A3R2; TEAD3; TEAD4; NKX2-1; RUNX1;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation: Relative Expression (Western Blot)
  
• Blocking Peptide: For anti-WWTR1 (ARP32540_P050) antibody is Catalog # AAP32540 (Previous Catalog # AAPP35146)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human WWTR1
• Uniprot ID: Q9GZV5
• Protein Name: WW domain-containing transcription regulator protein 1
• Protein Accession #: NP_056287
• Purification: Affinity Purified
• Nucleotide Accession #: NM_015472
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: WWTR1
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Image 1: Mouse Small Intestine
  
  Host: Rabbit
  Target Name: WWTR1
  Sample Tissue: Mouse Small Intestine
  Antibody Dilution: 0.5ug/ml
• Image 2: Mouse brain, Mouse intestine
  
  Host: Rabbit
  Target: WWTR1
  Positive control (+): Mouse brain (M-BR)
  Negative control (-): Mouse intestine (M-IN)
  Antibody concentration: 1ug/ml
• Image 3: Mouse Kidney
  
  Host: Mouse
  Target Name: WWTR1
  Sample Tissue: Mouse Kidney
  Antibody Dilution: 1ug/ml
• Image 4: Western Blot
  
  25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. Canonical 50 kDa isoform is identified.
• Image 5:
  
  Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.