Product Number |
ARP32537_P050 |
Product Page |
www.avivasysbio.com/pou2f3-antibody-n-terminal-region-arp32537-p050.html |
Name |
POU2F3 Antibody - N-terminal region (ARP32537_P050) |
Protein Size (# AA) |
436 amino acids |
Molecular Weight |
47 kDa |
NCBI Gene Id |
25833 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
POU class 2 homeobox 3 |
Alias Symbols |
PLA1, OCT11, PLA-1, Epoc-1, OCT-11, OTF-11, Skn-1a |
Peptide Sequence |
Synthetic peptide located within the following region: KMSGDVADSTDARSTLSQVEPGNDRKGLDFNRQIKTEDLSDSLQQTLSHR |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Enomoto,Y., et al., (2004) Oncogene 23 (29), 5014-5022 |
Description of Target |
POU domain genes encode a family of highly conserved transacting factors that influence the transcriptional activity of several cell type-specific and ubiquitous genes. |
Protein Interactions |
UBC; BCL6; CREBBP; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Enhanced Validation |
Relative Expression (Western Blot) |
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Datasheets/Manuals |
Printable datasheet for anti-POU2F3 (ARP32537_P050) antibody |
Blocking Peptide |
For anti-POU2F3 (ARP32537_P050) antibody is Catalog # AAP32537 (Previous Catalog # AAPP03540) |
Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human POU2F3 |
Uniprot ID |
Q9UKI9 |
Protein Name |
POU domain, class 2, transcription factor 3 |
Protein Accession # |
NP_055167 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_014352 |
Tested Species Reactivity |
Human, Mouse, Rat |
Gene Symbol |
POU2F3 |
Predicted Species Reactivity |
Human, Mouse, Rat, Dog, Guinea Pig, Rabbit, Sheep, Zebrafish |
Application |
IHC, WB |
Predicted Homology Based on Immunogen Sequence |
Dog: 100%; Guinea Pig: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 77% |
Image 1 | Rat Brain
| Host: Rat Target Name: POU2F3 Sample Tissue: Rat Brain Antibody Dilution: 1ug/ml |
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Image 2 | Human Muscle
| Human Muscle |
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Image 3 | Human Skin
| Rabbit Anti-POU2F3 antibody Catalog Number: ARP32537 Formalin Fixed Paraffin Embedded Tissue: Human Skin Primary antibody Concentration: 1:200 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20x Exposure Time: 0.5-2.0sec
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Image 4 | Mouse tongue
| Sample Type: Mouse tongue tissuePrimary Antibody Dilution: 1:100Secondary Antibody: Anti-rabbit-Cy3Secondary Antibody Dilution: 1:500Color/Signal Descriptions: Red: POU2F3Gene Name: POU2F3Submitted by: Dr. Hong Wang, Monell Chemical Senses Center |
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Image 5 | Human HepG2
| WB Suggested Anti-POU2F3 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positive Control: HepG2 cell lysate |
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Image 6 | Western Blot
| 25 ug of the indicated whole cell extracts was loaded onto a 12% SDS-PAGE gel. 0.5 ug/mL of the antibody was used in this experiment. N-terminal peptide also overlaps with isoform 3 at 39 kDa in some samples. |
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