Hey1 Antibody - C-terminal region (ARP32513_P050)

Data Sheet
 
Product Number ARP32513_P050
Product Page www.avivasysbio.com/hey1-antibody-c-terminal-region-arp32513-p050.html
Name Hey1 Antibody - C-terminal region (ARP32513_P050)
Protein Size (# AA) 292 amino acids
Molecular Weight 32 kDa
NCBI Gene Id 155437
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Hairy/enhancer-of-split related with YRPW motif 1
Alias Symbols Herp2
Peptide Sequence Synthetic peptide located within the following region: WGSAFGHHPHIAHPLLLPQNGHGNAGTTASPTEPHHQGRLASAHPEAPAL
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Description of Target Hey1 is a primary target of Notch that plays a central role in the development of the aorta.
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-Hey1 (ARP32513_P050) antibody
Blocking Peptide For anti-Hey1 (ARP32513_P050) antibody is Catalog # AAP32513 (Previous Catalog # AAPP03516)
Uniprot ID D3ZNI5
Protein Name Protein Hey1 Ensembl ENSRNOP00000015537
Protein Accession # NP_001178774
Purification Affinity Purified
Nucleotide Accession # NM_001191845
Tested Species Reactivity Rat
Gene Symbol Hey1
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse
Application WB
Predicted Homology Based on Immunogen Sequence Cow: 79%; Dog: 79%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 93%; Rat: 100%
Image 1
Rat Muscle
WB Suggested Anti-Hey1 Antibody
Titration: 1.0 ug/ml
Positive Control: Rat Muscle
Image 2

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
 

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