| Product Number |
ARP32457_T100 |
| Product Page |
www.avivasysbio.com/polr2b-antibody-n-terminal-region-arp32457-t100.html |
| Name |
POLR2B Antibody - N-terminal region (ARP32457_T100) |
| Protein Size (# AA) |
1174 amino acids |
| Molecular Weight |
134kDa |
| Subunit |
RPB2 |
| NCBI Gene Id |
5431 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1.0 mg/ml |
| Gene Full Name |
Polymerase (RNA) II (DNA directed) polypeptide B, 140kDa |
| Alias Symbols |
RPB2, POL2RB, hRPB140 |
| Peptide Sequence |
Synthetic peptide located within the following region: TYSAPLYVDITKTVIKEGEEQLQTQHQKTFIGKIPIMLRSTYCLLNGLTD |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Langelier,M.F., et al., (2005) (er) Nucleic Acids Res. 33 (8), 2629-2639 |
| Description of Target |
POLR2B is the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA. |
| Protein Interactions |
ESR1; UBC; TCEB3; SRC; PSMB9; SUMO2; XRN2; WWOX; SUZ12; RNF2; NEDD4; ITCH; RABEP2; HECW2; FBXO6; CDK20; HDAC11; ILK; POLR2C; MED19; MED26; RECQL5; VCP; BARD1; MDC1; POLR3F; TUBB4B; TUBA4A; POLR2I; POLR2E; POLR2D; POLR2A; DCTN1; ATP6V1B2; MEN1; CDK7; CCNH; |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-POLR2B (ARP32457_T100) antibody |
| Blocking Peptide |
For anti-POLR2B (ARP32457_T100) antibody is Catalog # AAP32457 (Previous Catalog # AAPP03453) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human POLR2B |
| Uniprot ID |
P30876 |
| Protein Name |
DNA-directed RNA polymerase II subunit RPB2 |
| Protein Accession # |
NP_000929 |
| Purification |
Protein A purified |
| Nucleotide Accession # |
NM_000938 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
POLR2B |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
| Application |
WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 85% |
| Image 1 | Human HepG2
 | WB Suggested Anti-POLR2B Antibody Titration: 5ug/ml
Positive Control: HepG2 cell lysate |
|
