PAX7 Antibody - N-terminal region (ARP32393_P050)

• Product Number: ARP32393_P050
• Name: PAX7 Antibody - N-terminal region (ARP32393_P050)
• Protein Size (# AA): 520 amino acids
• Molecular Weight: 55 kDa
• NCBI Gene Id: 5081
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Paired box 7
• Alias Symbols: HUP1, RMS2, PAX7B, MYOSCO
• Peptide Sequence: Synthetic peptide located within the following region: KEEEDEADKKEDDGEKKAKHSIDGILGDKGNRLDEGSDVESEPDLPLKRK
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Du,S., et al., (2005) Int. J. Cancer 115 (1), 85-92
• Description of Target: PAX7 is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box gene 7 is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Alternative splicing in this gene has produced two known products but the biological significance of the variants is unknown.
• Protein Interactions: TRIM27; MYOD1; Dlg4; WDR5; ASH2L; HIRA; Ubc;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation:

  WB	Y
  SPR
  YCHAROS

• Blocking Peptide: For anti-PAX7 (ARP32393_P050) antibody is Catalog # AAP32393 (Previous Catalog # AAPP03383)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PAX7
• Uniprot ID: P23759
• Protein Name: Paired box protein Pax-7
• Protein Accession #: NP_002575
• Purification: Affinity Purified
• Nucleotide Accession #: NM_002584
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: PAX7
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Zebrafish
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93%
• Image 1: Human Jurkat
  
  WB Suggested Anti-PAX7 Antibody Titration: 0.05ug/ml
  Positive Control: Jurkat cell lysate
• Image 2: Mouse skeletal muscle
  
  Lanes:
  1: Mouse skeletal muscle lysate, 2: Mouse skeletal muscle lysate, 3: Mouse skeletal muscle lysate
  Primary Antibody Dilution:
  1:1000
  Secondary Antibody:
  Anti-rabbit HRP
  Secondary Antibody Dilution:
  1:10000
  Gene Name:
  PAX7
  Submitted by:
  Anonymous
  
• Image 3: Human Skin
  
  Immunohistochemistry with Human Skin lysate tissue at an antibody concentration of 5.0ug/ml using anti-PAX7 antibody (ARP32393_P050)
• Image 4: Human kidney
  
  Rabbit Anti-PAX7 Antibody
  Catalog Number: ARP32393
  Paraffin Embedded Tissue: Human Kidney
  Cellular Data: Epithelial cells of renal tubule
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X