ASCL1 Antibody - N-terminal region (ARP32355_T100)

Data Sheet
 
Product Number ARP32355_T100
Product Page www.avivasysbio.com/ascl1-antibody-n-terminal-region-arp32355-t100.html
Name ASCL1 Antibody - N-terminal region (ARP32355_T100)
Protein Size (# AA) 236 amino acids
Molecular Weight 25 kDa
NCBI Gene Id 429
Host Rabbit
Clonality Polyclonal
Concentration 1.0 mg/ml
Gene Full Name Achaete-scute complex homolog 1 (Drosophila)
Alias Symbols ASH1, HASH1, MASH1, bHLHa46
Peptide Sequence Synthetic peptide located within the following region: QSAQQQQQQQQQQQQAPQLRPAADGQPSGGGHKSAPKQVKRQRSSSPELM
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Somasundaram,K., et al., (2005) Oncogene. 24(47), 7073-83
Description of Target ASCL1 encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. It is highly expressed in medullary thyroid cancer and small cell lung cancer and may be a useful marker for these cancers.
Protein Interactions MEF2D; MEF2C; MEF2A; NEUROG2; UBQLN1; EP300; TCF4; BMP2; ASCL1;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Enhanced Validation
Relative Expression (Western Blot) Avivasheild
Datasheets/Manuals Printable datasheet for anti-ASCL1 (ARP32355_T100) antibody
Blocking Peptide For anti-ASCL1 (ARP32355_T100) antibody is Catalog # AAP32355 (Previous Catalog # AAPP03344)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human ASCL1
Uniprot ID P50553
Protein Name Achaete-scute homolog 1
Publications

Suozzi, A., Malatesta, M. & Zancanaro, C. Subcellular distribution of key enzymes of lipid metabolism during the euthermia-hibernation-arousal cycle. J. Anat. 214, 956-62 (2009). 19538638

Vias, M. et al. Pro-neural transcription factors as cancer markers. BMC Med. Genomics 1, 17 (2008). 18489756

Protein Accession # NP_004307
Purification Protein A purified
Nucleotide Accession # NM_004316
Tested Species Reactivity Human
Gene Symbol ASCL1
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 92%; Guinea Pig: 100%; Human: 100%; Mouse: 92%; Rabbit: 92%; Rat: 92%
Image 1
Human kidney
Human kidney
Image 2
Human Thymus
WB Suggested Anti-ASCL1 Antibody Titration: 1.25ug/ml
Positive Control: Human Thymus
Image 3

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 4 ug/mL of the antibody was used in this experiment.
 

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