Product Number |
ARP32355_T100 |
Product Page |
www.avivasysbio.com/ascl1-antibody-n-terminal-region-arp32355-t100.html |
Name |
ASCL1 Antibody - N-terminal region (ARP32355_T100) |
Protein Size (# AA) |
236 amino acids |
Molecular Weight |
25 kDa |
NCBI Gene Id |
429 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1.0 mg/ml |
Gene Full Name |
Achaete-scute complex homolog 1 (Drosophila) |
Alias Symbols |
ASH1, HASH1, MASH1, bHLHa46 |
Peptide Sequence |
Synthetic peptide located within the following region: QSAQQQQQQQQQQQQAPQLRPAADGQPSGGGHKSAPKQVKRQRSSSPELM |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Somasundaram,K., et al., (2005) Oncogene. 24(47), 7073-83 |
Description of Target |
ASCL1 encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. It is highly expressed in medullary thyroid cancer and small cell lung cancer and may be a useful marker for these cancers. |
Protein Interactions |
MEF2D; MEF2C; MEF2A; NEUROG2; UBQLN1; EP300; TCF4; BMP2; ASCL1; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Enhanced Validation |
Relative Expression (Western Blot) |
|
|
Datasheets/Manuals |
Printable datasheet for anti-ASCL1 (ARP32355_T100) antibody |
Blocking Peptide |
For anti-ASCL1 (ARP32355_T100) antibody is Catalog # AAP32355 (Previous Catalog # AAPP03344) |
Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human ASCL1 |
Uniprot ID |
P50553 |
Protein Name |
Achaete-scute homolog 1 |
Publications |
Suozzi, A., Malatesta, M. & Zancanaro, C. Subcellular distribution of key enzymes of lipid metabolism during the euthermia-hibernation-arousal cycle. J. Anat. 214, 956-62 (2009). 19538638
Vias, M. et al. Pro-neural transcription factors as cancer markers. BMC Med. Genomics 1, 17 (2008). 18489756 |
Protein Accession # |
NP_004307 |
Purification |
Protein A purified |
Nucleotide Accession # |
NM_004316 |
Tested Species Reactivity |
Human |
Gene Symbol |
ASCL1 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit |
Application |
IHC, WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 92%; Guinea Pig: 100%; Human: 100%; Mouse: 92%; Rabbit: 92%; Rat: 92% |
Image 1 | Human kidney
| Human kidney |
|
Image 2 | Human Thymus
| WB Suggested Anti-ASCL1 Antibody Titration: 1.25ug/ml Positive Control: Human Thymus |
|
Image 3 |
| 25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 4 ug/mL of the antibody was used in this experiment.
|
|