ERCC8 Antibody - N-terminal region (ARP32149_P050)

• Product Number: ARP32149_P050
• Name: ERCC8 Antibody - N-terminal region (ARP32149_P050)
• Protein Size (# AA): 396 amino acids
• Molecular Weight: 44kDa
• NCBI Gene Id: 1161
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Excision repair cross-complementing rodent repair deficiency, complementation group 8
• Alias Symbols: CSA, CKN1, UVSS2
• Peptide Sequence: Synthetic peptide located within the following region: DVERIHGGGINTLDIEPVEGRYMLSGGSDGVIVLYDLENSSRQSYYTCKA
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Sarker,A.H., et al., (2005) Mol. Cell 20 (2), 187-198
• Description of Target: ERCC8 is a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). This gene encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes.
• Protein Interactions: CUL4A; CUL4B; DDB1; RUVBL2; ZNF24; PCNA; H1F0; ERCC6; UVSSA; DDA1; RFWD2; COPS4; COPS6; COPS3; TOP1; TP53; SSBP1; OGG1; MDM2; GTF2H2; CUL5; GPS1; DDB2; COPS2; COPS7A; COPS5; COPS8; RBX1; POLR2A; HMGN1; EP300; UBC; CSNK2B; XAB2; UQCRQ; CBR1;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-ERCC8 (ARP32149_P050) antibody is Catalog # AAP32149 (Previous Catalog # AAPP03115)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ERCC8
• Uniprot ID: Q13216
• Protein Name: DNA excision repair protein ERCC-8
• Protein Accession #: NP_000073
• Purification: Affinity Purified
• Nucleotide Accession #: NM_000082
• Tested Species Reactivity: Human
• Gene Symbol: ERCC8
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Pig, Rabbit, Zebrafish
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Cow: 77%; Dog: 85%; Guinea Pig: 77%; Human: 100%; Mouse: 85%; Pig: 85%; Rabbit: 85%; Rat: 77%; Zebrafish: 85%
• Image 1: Human Muscle
  
  WB Suggested Anti-ERCC8 Antibody Titration: 0.2-1 ug/ml
  Positive Control: Human muscle
• Image 2: Human liver
  
  WB Suggested Anti-ERCC8 antibody Titration: 1 ug/mL
  Sample Type: Human liver
• Image 3: liver
  
  Rabbit Anti-ERCC8 Antibody
  Catalog Number: ARP32149_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Adult liver
  Observed Staining: Cytoplasmic,Nuclear
  Primary Antibody Concentration: 1:600
  Secondary Antibody: Donkey anti-Rabbit-Cy2/3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 – 2.0 sec
  Protocol located in Reviews and Data.