| Product Number |
ARP32062_P050 |
| Product Page |
www.avivasysbio.com/pawr-antibody-middle-region-arp32062-p050.html |
| Name |
PAWR Antibody - middle region (ARP32062_P050) |
| Protein Size (# AA) |
340 amino acids |
| Molecular Weight |
37kDa |
| NCBI Gene Id |
5074 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
PRKC, apoptosis, WT1, regulator |
| Alias Symbols |
PAR4, Par-4 |
| Peptide Sequence |
Synthetic peptide located within the following region: VNIPAAECLDEYEDDEAGQKERKREDAITQQNTIQNEAVNLLDPGSSYLL |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Bergmann,M., et al., (2004) Ann.Hematol.83(10),646-653 |
| Description of Target |
The tumor suppressor WT1 represses and activates transcription. Anti-Prostate Apoptosis Response Protein Par-4 (PAWR) is a WT1-interacting protein that itself functions as a transcriptional repressor. It contains a putative leucine zipper domain which interacts with the zinc finger DNA binding domain of WT1. This protein is specifically upregulated during apoptosis of prostate cells. |
| Protein Interactions |
FBXO45; UBC; EED; FAM129B; SNX6; CAND1; VPS29; JMJD6; STAT1; SNX2; SHMT2; SHMT1; PPM1G; IDE; H3F3A; CARS; ATP6V1C1; APEH; PAN2; DRD2; DAPK3; SQSTM1; PRKCZ; Spsb4; Spsb2; SPSB1; FBXO25; HSPA5; Cep350; Kif23; THAP1; SLC5A7; AATF; TFPT; PAWR; WT1; RRAS2; ATP |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-PAWR (ARP32062_P050) antibody |
| Blocking Peptide |
For anti-PAWR (ARP32062_P050) antibody is Catalog # AAP32062 (Previous Catalog # AAPP02964) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human PAWR |
| Uniprot ID |
O75796 |
| Protein Name |
PRKC apoptosis WT1 regulator protein |
| Protein Accession # |
NP_002574 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_002583 |
| Tested Species Reactivity |
Human, Rat |
| Gene Symbol |
PAWR |
| Predicted Species Reactivity |
Human, Mouse, Rat, Guinea Pig, Horse, Rabbit |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 86%; Rat: 100% |
| Image 1 | Human Jurkat
 | WB Suggested Anti-PAWR Antibody Titration: 0.2-1 ug/ml
Positive Control: Jurkat cell lysate |
|
| Image 2 | Human Muscle
 | Human Muscle |
|
| Image 3 | rat striatum homogenate
 | WB Suggested Anti-PAWR Antibody
Positive Control: Lane 1: 30ug rat striatum homogenate
Primary Antibody Dilution : 1:1000
Secondary Antibody : Anti rabbit-HRP
Secondry Antibody Dilution : 1:10,000
Submitted by: Kristy Shimp, University of Florida |
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