MBD1 Antibody - C-terminal region (ARP32012_P050)

• Product Number: ARP32012_P050
• Name: MBD1 Antibody - C-terminal region (ARP32012_P050)
• Protein Size (# AA): 549 amino acids
• Molecular Weight: 60kDa
• NCBI Gene Id: 4152
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Methyl-CpG binding domain protein 1
• Alias Symbols: RFT, PCM1, CXXC3
• Peptide Sequence: Synthetic peptide located within the following region: NKDDSASKLAPEEEAGGAGTPVITEIFSLGGTRFRDTAVWLPRSKDLKKP
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Villa,R., (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (5), 1400-1405
• Description of Target: MBD1 belongs to a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD1 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters; in addition, variants with three CXXC domains also repress unmethylated promoter activity. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters; in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21.DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters; in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21.
• Protein Interactions: SUMO1; HTT; UBC; ECT2; APP; ATXN1L; TENM1; SUMO2; HIST1H3A; PCNA; ATF7IP2; ATF7IP; CHAF1A; SETDB1; PIAS1; STAT1; PRAM1; HDAC3; PIAS2; ZNF512B; Rnf2; CBX4; CBX5; HIPK3; SMAD9; OASL; SMAD5; SMAD3; SMAD7; SMAD1; SUV39H1;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-MBD1 (ARP32012_P050) antibody is Catalog # AAP32012 (Previous Catalog # AAPP23942)
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human MBD1
• Uniprot ID: Q9UIS9-3
• Protein Name: Methyl-CpG-binding domain protein 1
• Publications:

  Jungwirth, M., Dear, M. L., Brown, P., Holbrook, K. & Goodchild, R. Relative tissue expression of homologous torsinB correlates with the neuronal specific importance of DYT1 dystonia-associated torsinA. Hum. Mol. Genet. 19, 888-900 (2010). 20034025

• Protein Accession #: NP_056669
• Purification: Affinity Purified
• Nucleotide Accession #: NM_015844
• Tested Species Reactivity: Human
• Gene Symbol: MBD1
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 86%; Rabbit: 100%; Rat: 100%
• Image 1: Human HepG2
  
  WB Suggested Anti-MBD1 Antibody Titration: 0.2-1 ug/ml
  Positive Control: HepG2 cell lysate