| Product Number |
ARP32006_P050 |
| Product Page |
www.avivasysbio.com/smad5-antibody-middle-region-arp32006-p050.html |
| Name |
SMAD5 Antibody - middle region (ARP32006_P050) |
| Protein Size (# AA) |
465 amino acids |
| Molecular Weight |
51kDa |
| NCBI Gene Id |
4090 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
SMAD family member 5 |
| Alias Symbols |
DWFC, JV5-1, MADH5 |
| Peptide Sequence |
Synthetic peptide located within the following region: YPPSPASSTYPNSPASSGPGSPFQLPADTPPPAYMPPDDQMGQDNSQPMD |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Zimonjic,D.B., et al., (2003) Neoplasia 5 (5), 390-396 |
| Description of Target |
SMAD5 undergoes copy number gain and increased expression, rather than loss of expression, and therefore does not act as a tumor-suppressor gene in hepatocellular carcinoma. Up-regulated Smad5 mediates apoptosis of gastric epithelial cells induced by Helicobacter pylori infection. |
| Protein Interactions |
UBC; SUMO1; HECW2; HBG2; BCAT1; SOX5; SNRPA; SNRNP70; SFPQ; RYR2; RBM4; PTPN12; PSMD11; NEDD4; SMAD4; SMAD1; ANKRD13A; PPIL4; SOX7; SMURF2; SMURF1; MTMR10; CHMP3; CXXC5; SF3B1; U2AF2; PNKP; WWP1; NAA20; EPN1; SMAD3; SMAD2; PAX6; COPS5; HGS; EP300; ELAVL1; |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-SMAD5 (ARP32006_P050) antibody |
| Blocking Peptide |
For anti-SMAD5 (ARP32006_P050) antibody is Catalog # AAP32006 (Previous Catalog # AAPP02907) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human SMAD5 |
| Uniprot ID |
Q99717 |
| Protein Name |
Mothers against decapentaplegic homolog 5 |
| Protein Accession # |
NP_005894 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_005903 |
| Tested Species Reactivity |
Human, Rat |
| Gene Symbol |
SMAD5 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish |
| Application |
WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 93%; Zebrafish: 93% |
| Image 1 | Human HepG2
 | WB Suggested Anti-SMAD5 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: HepG2 cell lysate |
|
| Image 2 | r-SMAD5
 | Lanes:
Lane 1: 5ug of transfected 293T lysate (SMAD1)
Lane 1: 5ug of transfected 293T lysate (SMAD2)
Lane 1: 5ug of transfected 293T lysate (SMAD3)
Lane 1: 5ug of transfected 293T lysate (SMAD4)
Lane 1: 5ug of transfected 293T lysate (SMAD5)
Lane 1: 5ug of transfected 293T lysate (SMAD6)
Lane 1: 5ug of transfected 293T lysate (SMAD7)
Lane 1: 5ug of transfected 293T lysate (SMAD8)
Lane 1: 5ug of transfected 293T lysate (GFP)
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Goat anti-Rabbit IgG HRP Conjugated
Secondary Antibody Dilution:
1:10,000
Gene Name:
SMAD5
Submitted by:
Amanda Urick, Medical College of Wisconsin
|
|
| Image 3 | Rat Liver
 | Host: Rat
Target Name: SMAD5
Sample Tissue: Rat Liver
Antibody Dilution: 1ug/ml |
|
