| Product Number |
ARP32005_T100 |
| Product Page |
www.avivasysbio.com/smad4-antibody-middle-region-arp32005-t100.html |
| Name |
SMAD4 Antibody - middle region (ARP32005_T100) |
| Protein Size (# AA) |
552 amino acids |
| Molecular Weight |
60 kDa |
| NCBI Gene Id |
4089 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1.0 mg/ml |
| Gene Full Name |
SMAD family member 4 |
| Alias Symbols |
JIP, DPC4, MADH4, MYHRS |
| Peptide Sequence |
Synthetic peptide located within the following region: NIPVASTSQPASILGGSHSEGLLQIASGPQPGQQQNGFTGQPATYHHNST |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Kantola,A.K., et al., Gene 363, 142-150 (2005) |
| Description of Target |
SMAD4 is one of the Smad family members, which are essential intracellular signalling components of the transforming growth factor-beta (TGF-beta) superfamily. Smad2 and Smad3 are structurally highly similar and mediate TGF-beta signals. Smad4 is distantly related to Smads 2 and 3, and forms a heteromeric complex with Smad2 after TGF-beta or activin stimulation. TGF-beta induces heteromeric complexes of Smads 2, 3 and 4, and their concomitant translocation to the nucleus, which is required for efficient TGF-beta signal transduction |
| Protein Interactions |
RASSF5; LMO4; SMAD3; SMAD2; SKIL; UBC; NEDD8; SUMO1; SMURF2; SMURF1; TRIM33; CRYAB; COPS5; NLK; OTUB1; SMAD9; UBE2I; FHL3; FHL2; FHL1; CSNK1D; TGFB1I1; SMAD1; NCOR1; SKI; KLF5; IRF7; IRF3; ZNF521; ZNF423; SOX12; SNRNP70; RPL28; RFX1; RELA; RALA; PSMD11; P |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Enhanced Validation |
| Relative Expression (Western Blot) |
 |
|
| Datasheets/Manuals |
Printable datasheet for anti-SMAD4 (ARP32005_T100) antibody |
| Blocking Peptide |
For anti-SMAD4 (ARP32005_T100) antibody is Catalog # AAP32005 (Previous Catalog # AAPP02906) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human SMAD4 |
| Uniprot ID |
Q13485 |
| Protein Name |
Mothers against decapentaplegic homolog 4 |
| Protein Accession # |
NP_005350 |
| Purification |
Protein A purified |
| Nucleotide Accession # |
NM_005359 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
SMAD4 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Sheep |
| Application |
WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 92%; Goat: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 92%; Rabbit: 100%; Rat: 100%; Sheep: 100% |
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