GATA2 Antibody - N-terminal region (ARP31855_T100)

• Product Number: ARP31855_T100
• Name: GATA2 Antibody - N-terminal region (ARP31855_T100)
• Protein Size (# AA): 480 amino acids
• Molecular Weight: 51 kDa
• NCBI Gene Id: 2624
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: GATA binding protein 2
• Alias Symbols: DCML, IMD21, NFE1B, MONOMAC
• Peptide Sequence: Synthetic peptide located within the following region: PYYANPAHARARVSYSPAHARLTGGQMCRPHLLHSPGLPWLDGGKAALSA
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Tsuzuki, S., et al., (2004) Mol. Cell. Biol. 24 (15), 6824-6836
• Description of Target: The GATA family of transcription factors, which contain zinc fingers in their DNA binding domain, have emerged as candidate regulators of gene expression in hematopoietic cells is essential for normal primitive and definitive erythropoiesis and is expressed at high levels in erythroid cells, mast cells, and megakaryocytes. GATA2 is expressed in hematopoietic progenitors, including early erythroid cells, mast cells, and megakaryocytes, and also in nonhematopoietic embryonic stem cells.
• Protein Interactions: NOTCH2NL; KRTAP10-3; KRTAP10-9; KRT40; PRR20A; ADAMTSL4; TRAF1; PSMA3; MDFI; GOLGA2; FHL3; TRIM23; AKT1; BMI1; SPI1; SMAD4; ELAVL1; KAT2A; EP300; MED1; POU1F1; POU2AF1; ZBTB32; ZFPM1; ZBTB16; HDAC5; HDAC3; HHEX; RARA; RXRA; MAPK1; JUN; CEBPA; STAT3; PML;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation: Relative Expression (Western Blot)
  
• Blocking Peptide: For anti-GATA2 (ARP31855_T100) antibody is Catalog # AAP31855 (Previous Catalog # AAPP02650)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GATA2
• Uniprot ID: P23769
• Protein Name: Endothelial transcription factor GATA-2
• Publications:

  Fujiwara, T. et al. Gene expression profiling identifies HOXB4 as a direct downstream target of GATA-2 in human CD34+ hematopoietic cells. PLoS One 7, e40959 (2012). 23028422

  Huang, Y.-J. et al. The functional IGFBP7 promoter -418G>A polymorphism and risk of head and neck cancer. Mutat. Res. 702, 32-9 (2010). 20599521

  Jack, B. H. A. & Crossley, M. GATA proteins work together with friend of GATA (FOG) and C-terminal binding protein (CTBP) co-regulators to control adipogenesis. J. Biol. Chem. 285, 32405-14 (2010). 20705609

  Mammoto, A. et al. A mechanosensitive transcriptional mechanism that controls angiogenesis. Nature 457, 1103-8 (2009). 19242469

  Wang, F. et al. A regulatory circuit comprising GATA1/2 switch and microRNA-27a/24 promotes erythropoiesis. Nucleic Acids Res. 42, 442-57 (2014). 24049083

• Sample Type Confirmation:

  GATA2 is strongly supported by BioGPS gene expression data to be expressed in K562

• Protein Accession #: NP_116027
• Purification: Protein A purified
• Nucleotide Accession #: NM_032638
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: GATA2
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Image 1: Human Liver
  
  Human Liver
• Image 2: Mouse liver, N2a
  
  Lane 1: 30ug mouse liver lysateLane 2: 30ug mouse N2a cell lysate
  Primary Antibody Dilution:
  1:500
  Secondary Antibody:
  Anti-rabbit-HRP
  Secondary Antibody Dilution:
  1:2500
  Gene Name:
  GATA2
  Submitted by:
  A Kalyani and Vinayak Gupta IIT Madras
• Image 3: Human K562
  
  WB Suggested Anti-GATA2 Antibody Titration: 1.0-2.0ug/ml
  ELISA Titer: 1:1562500
  Positive Control: K562 cell lysateGATA2 is strongly supported by BioGPS gene expression data to be expressed in Human K562 cells
• Image 4:
  
  25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment.