SOX2 Antibody - N-terminal region (ARP31737_P050)

• Product Number: ARP31737_P050
• Name: SOX2 Antibody - N-terminal region (ARP31737_P050)
• Protein Size (# AA): 317 amino acids
• Molecular Weight: 34kDa
• NCBI Gene Id: 6657
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: SRY (sex determining region Y)-box 2
• Alias Symbols: ANOP3, MCOPS3
• Peptide Sequence: Synthetic peptide located within the following region: MYNMMETELKPPGPQQTSGGGGGNSTAAAAGGNQKNSPDRVKRPMNAFMV
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Wang,P., (2008) Arch. Ophthalmol. 126 (5), 709-713
• Description of Target: SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in this gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation. This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Protein Interactions: SUMO1; DMAP1; TBX2; SETD7; FAM21A; FZR1; MMS19; MRPS35; ZNF462; LSM2; CCAR2; MIER1; ZDBF2; CHD8; EP400; TSHZ3; RANBP10; ZNF687; XPO5; INTS2; MTA3; CORO1B; CASC5; MRPS22; KCMF1; MEPCE; TRERF1; INTS9; PARVA; SCYL2; CHD7; ZCCHC8; SOX6; BRIX1; SAMD4B; GID8; C
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Additional Information: IHC Information: Kidney
  IHC Information: Thymus
  IHC Information: Spleen
• Blocking Peptide: For anti-SOX2 (ARP31737_P050) antibody is Catalog # AAP31737 (Previous Catalog # AAPP02528)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SOX2
• Uniprot ID: P48431
• Protein Name: Transcription factor SOX-2
• Publications:

  Perry, K. J., Thomas, A. G. & Henry, J. J. Expression of pluripotency factors in larval epithelia of the frog Xenopus: evidence for the presence of cornea epithelial stem cells. Dev. Biol. 374, 281-94 (2013). 23274420

• Sample Type Confirmation:

  There is BioGPS gene expression data showing that SOX2 is expressed in OVCAR3

• Protein Accession #: NP_003097
• Purification: Affinity Purified
• Nucleotide Accession #: NM_003106
• Tested Species Reactivity: Human, Mouse, Frog
• Gene Symbol: SOX2
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Horse, Pig, Rabbit, Sheep, Zebrafish
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Goat: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 91%
• Image 1: Human Spleen
  
  Human Spleen
• Image 2: Human Kidney
  
  Human Kidney
• Image 3: Xenopus laevis cornea epithellium
  
  Researcher:Kim Perry, University of Illinois Application:IHC Species+tissue/cell type:Xenopus laevis cornea epithellium Primary antibody dilution:1:300 Secondary antibody:Goat anti-rabbit -rhodamine Secondary antibody dilution:1:300
• Image 4: Human 293T
  
  Host: Rabbit
  Target Name: SOX2
  Sample Tissue: Human 293T
  Antibody Dilution: 1.0ug/ml
• Image 5: Mouse Liver
  
  Host: Mouse
  Target Name: SOX2
  Sample Tissue: Mouse Liver
  Antibody Dilution: 1ug/ml
• Image 6: Mouse Pancreas
  
  Host: Mouse
  Target Name: SOX2
  Sample Tissue: Mouse Pancreas
  Antibody Dilution: 1ug/ml
• Image 7: Mouse Liver
  
  Host: Rabbit
  Target Name: SOX2
  Sample Tissue: Mouse Liver
  Antibody Dilution: 1ug/ml
• Image 8: U87
  
  Sample Type: Lane 1: 20 ug U87 lysate
  Primary Antibody Dilution: 1:1000
  Secondary Antibody: Anti-rabbit-HRP
  Secondary Antibody Dilution: 1:2000
  Submitted by: Ander Matheu Fernandez, Biodonostia Institute