Product Number |
ARP31692_P050 |
Product Page |
www.avivasysbio.com/trim32-antibody-c-terminal-region-arp31692-p050.html |
Name |
TRIM32 Antibody - C-terminal region (ARP31692_P050) |
Protein Size (# AA) |
653 amino acids |
Molecular Weight |
72kDa |
NCBI Gene Id |
22954 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Tripartite motif containing 32 |
Description |
|
Alias Symbols |
HT2A, BBS11, TATIP, LGMD2H, LGMDR8 |
Peptide Sequence |
Synthetic peptide located within the following region: GFSIGSVGPDGQLGRQISHFFSENEDFRCIAGMCVDARGDLIVADSSRKE |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Chiang,A.P., (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (16), 6287-6292 |
Description of Target |
TRIM32 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. TRIM32 localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes. |
Protein Interactions |
PTPN11; PDE9A; PTCD2; CLIP4; KCTD9; UBQLN1; TRIM32; RABAC1; ABI2; SCGB1A1; UBE2E1; UBE2D1; UBC; TP53; SDCBP; UBE2U; SYT6; MYCN; CEP250; CUL3; GPRASP2; GLIS2; YWHAB; TP73; UBE2D3; UBE2D2; HSP90AA1; SFN; YWHAQ; YWHAZ; YWHAH; YWHAG; YWHAE; NOTCH1; UBD; TOP1; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-TRIM32 (ARP31692_P050) antibody |
Blocking Peptide |
For anti-TRIM32 (ARP31692_P050) antibody is Catalog # AAP31692 (Previous Catalog # AAPP02479) |
Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human TRIM32 |
Uniprot ID |
Q13049 |
Protein Name |
E3 ubiquitin-protein ligase TRIM32 |
Publications |
Deficiency of the E3 ubiquitin ligase TRIM32 in mice leads to a myopathy with a neurogenic component. Hum Mol Genet. 18, 1353-67 (2009). 19155210 |
Sample Type Confirmation |
TRIM32 is supported by BioGPS gene expression data to be expressed in HEK293T |
Protein Accession # |
NP_036342 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_012210 |
Tested Species Reactivity |
Human |
Gene Symbol |
TRIM32 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
Application |
IHC, WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 85% |
Image 1 | Human 293T
| WB Suggested Anti-TRIM32 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive Control: Transfected 293TTRIM32 is supported by BioGPS gene expression data to be expressed in HEK293T |
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Image 2 | Human Bronchial Epithelial Tissue
| Rabbit Anti-TRIM32 Antibody Catalog Number: ARP31692_P050 Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue Observed Staining: Cytoplasmic Primary Antibody Concentration: 1:100 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec
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Image 3 | Human HCT116 Whole Cell
| Host: Rabbit Target Name: TRIM32 Sample Tissue: Human HCT116 Whole Cell Antibody Dilution: 1ug/ml |
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