TRIM32 Antibody - C-terminal region (ARP31692_P050)

Data Sheet
 
Product Number ARP31692_P050
Product Page www.avivasysbio.com/trim32-antibody-c-terminal-region-arp31692-p050.html
Name TRIM32 Antibody - C-terminal region (ARP31692_P050)
Protein Size (# AA) 653 amino acids
Molecular Weight 72kDa
NCBI Gene Id 22954
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Tripartite motif containing 32
Description
Alias Symbols HT2A, BBS11, TATIP, LGMD2H, LGMDR8
Peptide Sequence Synthetic peptide located within the following region: GFSIGSVGPDGQLGRQISHFFSENEDFRCIAGMCVDARGDLIVADSSRKE
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Chiang,A.P., (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (16), 6287-6292
Description of Target TRIM32 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. TRIM32 localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. The protein has also been localized to the nucleus, where it interacts with the activation domain of the HIV-1 Tat protein. The Tat protein activates transcription of HIV-1 genes.
Protein Interactions PTPN11; PDE9A; PTCD2; CLIP4; KCTD9; UBQLN1; TRIM32; RABAC1; ABI2; SCGB1A1; UBE2E1; UBE2D1; UBC; TP53; SDCBP; UBE2U; SYT6; MYCN; CEP250; CUL3; GPRASP2; GLIS2; YWHAB; TP73; UBE2D3; UBE2D2; HSP90AA1; SFN; YWHAQ; YWHAZ; YWHAH; YWHAG; YWHAE; NOTCH1; UBD; TOP1;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-TRIM32 (ARP31692_P050) antibody
Blocking Peptide For anti-TRIM32 (ARP31692_P050) antibody is Catalog # AAP31692 (Previous Catalog # AAPP02479)
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human TRIM32
Uniprot ID Q13049
Protein Name E3 ubiquitin-protein ligase TRIM32
Publications

Deficiency of the E3 ubiquitin ligase TRIM32 in mice leads to a myopathy with a neurogenic component. Hum Mol Genet. 18, 1353-67 (2009). 19155210

Sample Type Confirmation

TRIM32 is supported by BioGPS gene expression data to be expressed in HEK293T

Protein Accession # NP_036342
Purification Affinity Purified
Nucleotide Accession # NM_012210
Tested Species Reactivity Human
Gene Symbol TRIM32
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 85%
Image 1
Human 293T
WB Suggested Anti-TRIM32 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Transfected 293TTRIM32 is supported by BioGPS gene expression data to be expressed in HEK293T
Image 2
Human Bronchial Epithelial Tissue
Rabbit Anti-TRIM32 Antibody
Catalog Number: ARP31692_P050
Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue
Observed Staining: Cytoplasmic
Primary Antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
Image 3
Human HCT116 Whole Cell
Host: Rabbit
Target Name: TRIM32
Sample Tissue: Human HCT116 Whole Cell
Antibody Dilution: 1ug/ml
 

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