ESRRG Antibody - middle region (ARP31655_T100)

• Product Number: ARP31655_T100
• Name: ESRRG Antibody - middle region (ARP31655_T100)
• Protein Size (# AA): 458 amino acids
• Molecular Weight: 51kDa
• NCBI Gene Id: 2104
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: Estrogen-related receptor gamma
• Alias Symbols: ERR3, ERRg, NR3B3, ERRgamma, ERR-gamma
• Peptide Sequence: Synthetic peptide located within the following region: RIDAENSPYLNPQLVQPAKKPYNKIVSHLLVAEPEKIYAMPDPTVPDSDI
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Hentschke,M., et al., (2003) Biochem. Biophys. Res. Commun. 312 (4), 975-982
• Description of Target: ERRs, which are coexpressed with ERs in prostatic cells, could regulate cell growth and modulate ER-mediated pathways via interference on ERalpha transcription in prostatic cells. Not only PNRC2 but also the corepressor TLE1 functioned as ERRgamma coactivator in a reporter gene analysis. Transcriptional activation by ERR3 can be ligand-independent.
• Protein Interactions: MEOX2; KIFC3; ESRRG; NR0B1; SUMO1P1; CCDC67; KIAA1598; NUP160; SRPK1; SRA1; PPARGC1A; APP; PNRC2; NR0B2; TLE1; ESRRA; EIF3I; NRIP1; SRC; PROX1; DUT; ARHGEF15; PNRC1; EBP; UBC; PARK2; DPF2; SP1; PPARGC1B; DAXX; NCOA1; CALM1; HGS;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-ESRRG (ARP31655_T100) antibody is Catalog # AAP31655 (Previous Catalog # AAPP02442)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ESRRG
• Uniprot ID: P62508
• Protein Name: Estrogen-related receptor gamma
• Publications:

  Song, G. & Wang, L. A conserved gene structure and expression regulation of miR-433 and miR-127 in mammals. PLoS One 4, e7829 (2009). 19946636

  Song, G. & Wang, L. Transcriptional mechanism for the paired miR-433 and miR-127 genes by nuclear receptors SHP and ERRgamma. Nucleic Acids Res. 36, 5727-35 (2008). 18776219

• Protein Accession #: NP_996318
• Purification: Protein A purified
• Nucleotide Accession #: NM_206595
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: ESRRG
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
• Image 1: Human Jurkat
  
  WB Suggested Anti-ESRRG Antibody Titration: 0.5ug/ml
  ELISA Titer: 1:62500
  Positive Control: Jurkat cell lysate
• Image 2: Human Lung
  
  Human Lung
• Image 3: Mouse Brain
  
  Host: Mouse
  Target Name: ESRRG
  Sample Tissue: Mouse Brain
  Antibody Dilution: 1ug/ml
• Image 4: Mouse kidney, Mouse small intestine
  
  Host: Rabbit
  Target: ESRRG
  Positive control (+): Mouse kidney (M-KI)
  Negative control (-): Mouse small intestine (M-IN)
  Antibody concentration: 3ug/ml
• Image 5: Jurkat
  
  Host: Rabbit
  Target Name: ESRRG
  Sample Type: Jurkat
  Lane A: Primary Antibody
  Lane B: Primary Antibody + Blocking Peptide
  Primary Antibody Concentration: 2.5ug/mL
  Peptide Concentration: 2.0ug/mL
  Lysate Quantity: 25ug/lane
  Gel Concentration: 12%