AEBP1 Antibody - N-terminal region (ARP31592_P050)

Data Sheet
 
Product Number ARP31592_P050
Product Page www.avivasysbio.com/aebp1-antibody-n-terminal-region-arp31592-p050.html
Name AEBP1 Antibody - N-terminal region (ARP31592_P050)
Protein Size (# AA) 1158 amino acids
Molecular Weight 131 kDa
NCBI Gene Id 165
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name AE binding protein 1
Alias Symbols ACLP
Peptide Sequence Synthetic peptide located within the following region: VPPEKTKDKGKKGKKDKGPKVPKESLEGSPRPPKKGKEKPPKATKKPKEK
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Description of Target The adipocyte enhancer binding protein 1 (AEBP1) is a transcriptional repressor with carboxypeptidase (CP) activity. This protein binds to a regulatory sequence, adipocyte enhancer 1 (AE-1), located in the proximal promoter region of the adipose P2 (aP2) gene, which encodes the adipocyte fatty-acid binding protein. It is characterized as a member of the regulatory B-like CP family. This protein seems to be activated by a novel mechanism, whereby the direct binding of DNA enhances its protease activity. AEBP1 may play a role in differentiated vascular smooth muscle cells.
Protein Interactions GRB2; TCF3;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Enhanced Validation
Relative Expression (Western Blot) Avivasheild
SPR Affinity Characterization Avivasheild
Datasheets/Manuals Printable datasheet for anti-AEBP1 (ARP31592_P050) antibody
Blocking Peptide For anti-AEBP1 (ARP31592_P050) antibody is Catalog # AAP31592 (Previous Catalog # AAPP02373)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human AEBP1
Uniprot ID Q14113
Protein Name Adipocyte enhancer-binding protein 1
Publications

Kim, H. J. et al. Identification of estradiol/ERa-regulated genes in the mouse pituitary. J. Endocrinol. 210, 309-21 (2011). 21700660

Protein Accession # NP_001120
Purification Affinity Purified
Nucleotide Accession # NM_001129
Tested Species Reactivity Human
Gene Symbol AEBP1
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Cow: 92%; Dog: 92%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 92%; Rabbit: 100%; Rat: 100%
Image 1
Human Intestine
Human Intestine
Image 2
Human Spermatophore
Human Spermatophore
Image 3
Western Blot
25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.
Image 4

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
 

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