Product Number |
ARP31592_P050 |
Product Page |
www.avivasysbio.com/aebp1-antibody-n-terminal-region-arp31592-p050.html |
Name |
AEBP1 Antibody - N-terminal region (ARP31592_P050) |
Protein Size (# AA) |
1158 amino acids |
Molecular Weight |
131 kDa |
NCBI Gene Id |
165 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
AE binding protein 1 |
Alias Symbols |
ACLP |
Peptide Sequence |
Synthetic peptide located within the following region: VPPEKTKDKGKKGKKDKGPKVPKESLEGSPRPPKKGKEKPPKATKKPKEK |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Description of Target |
The adipocyte enhancer binding protein 1 (AEBP1) is a transcriptional repressor with carboxypeptidase (CP) activity. This protein binds to a regulatory sequence, adipocyte enhancer 1 (AE-1), located in the proximal promoter region of the adipose P2 (aP2) gene, which encodes the adipocyte fatty-acid binding protein. It is characterized as a member of the regulatory B-like CP family. This protein seems to be activated by a novel mechanism, whereby the direct binding of DNA enhances its protease activity. AEBP1 may play a role in differentiated vascular smooth muscle cells. |
Protein Interactions |
GRB2; TCF3; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Enhanced Validation |
Relative Expression (Western Blot) |
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SPR Affinity Characterization |
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Datasheets/Manuals |
Printable datasheet for anti-AEBP1 (ARP31592_P050) antibody |
Blocking Peptide |
For anti-AEBP1 (ARP31592_P050) antibody is Catalog # AAP31592 (Previous Catalog # AAPP02373) |
Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human AEBP1 |
Uniprot ID |
Q14113 |
Protein Name |
Adipocyte enhancer-binding protein 1 |
Publications |
Kim, H. J. et al. Identification of estradiol/ERa-regulated genes in the mouse pituitary. J. Endocrinol. 210, 309-21 (2011). 21700660 |
Protein Accession # |
NP_001120 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_001129 |
Tested Species Reactivity |
Human |
Gene Symbol |
AEBP1 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit |
Application |
IHC, WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 92%; Dog: 92%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 92%; Rabbit: 100%; Rat: 100% |
Image 1 | Human Intestine
| Human Intestine |
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Image 2 | Human Spermatophore
| Human Spermatophore |
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Image 3 | Western Blot
| 25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. |
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Image 4 |
| Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
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