RIPK3 Antibody - N-terminal region (ARP31513_T100)

Data Sheet
 
Product Number ARP31513_T100
Product Page www.avivasysbio.com/ripk3-antibody-n-terminal-region-arp31513-t100.html
Name RIPK3 Antibody - N-terminal region (ARP31513_T100)
Protein Size (# AA) 518 amino acids
Molecular Weight 57 kDa
Conjugation Unconjugated
NCBI Gene Id 11035
Host Rabbit
Clonality Polyclonal
Concentration 1.0 mg/ml
Gene Full Name Receptor-interacting serine-threonine kinase 3
Description
Alias Symbols RIP3
Peptide Sequence Synthetic peptide located within the following region: GGSQSGTGSGEPGGTLGYLAPELFVNVNRKASTASDVYSFGILMWAVLAG
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Kimura,K., (2006) Genome Res. 16 (1), 55-65
Description of Target RIPK3 is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor.The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor.
Protein Interactions BMH1; RFX6; FADD; RIPK1; CASP8; INPP5K; CDC37; STIP1; PYGL; FKBP5; FKBP4; CTNND1; UBC; XIAP; BIRC3; BIRC2; TICAM1; TRAF2; TNFRSF1A;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Enhanced Validation
Relative Expression (Western Blot) Avivasheild
Datasheets/Manuals Printable datasheet for anti-RIPK3 (ARP31513_T100) antibody
Blocking Peptide For anti-RIPK3 (ARP31513_T100) antibody is Catalog # AAP31513 (Previous Catalog # AAPP24084)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human RIPK3
Uniprot ID Q9Y572
Protein Name Receptor-interacting serine/threonine-protein kinase 3
Publications

Mesenchymal Stem/Stromal Cells and their Extracellular Vesicle Progeny Decrease Injury in Poststenotic Swine Kidney Through Different Mechanisms. Stem Cells Dev. 29, 1190-1200 (2020)

Protein Accession # NP_006862
Purification Protein A purified
Nucleotide Accession # NM_006871
Tested Species Reactivity Human
Gene Symbol RIPK3
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Horse, Pig
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Cow: 79%; Dog: 93%; Horse: 86%; Human: 100%; Mouse: 86%; Pig: 86%; Rat: 91%
Image 1
Human Liver
Human Liver
Image 2
Human Heart
Human Heart
Image 3
Human Pancreas
RIPK3 antibody - N-terminal region (ARP31513_T100)
Catalog Number: ARP31513_T100
Formalin Fixed Paraffin Embedded Tissue: Human Pancreas Tissue
Observed Staining: Cytoplasmic in endocrine part (islets) of the pancreas. No staining observed in exocrine cells
Primary Antibody Concentration: 3 -12 ug/ml
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure: 0.2 seconds
Image 4
Western Blot
25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.
 

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