HDAC6 Antibody - N-terminal region (ARP31451_P050)

• Product Number: ARP31451_P050
• Name: HDAC6 Antibody - N-terminal region (ARP31451_P050)
• Protein Size (# AA): 1215 amino acids
• Molecular Weight: 131kDa, 17 kD
• NCBI Gene Id: 10013
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Histone deacetylase 6
• Alias Symbols: HD6, JM21, CPBHM, PPP1R90
• Peptide Sequence: Synthetic peptide located within the following region: VGLQGMDLNLEAEALAGTGLVLDEQLNEFHCLWDDSFPEGPERLHAIKEQ
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Int J Mol Med. 2003 Jul;12(1):87-93.
• Description of Target: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription.
• Protein Interactions: HSP90B1; ACTB; UBC; TRIM50; MYH10; DYNC1H1; DDIT3; ELAVL1; MAPK3; MAPK1; RUNX2; TUBA1B; SUMO1; RANBP9; NEDD8; ADRB2; ATXN3; MEF2B; SQSTM1; HSP90AA1; RASSF2; SLMAP; SAV1; CTTNBP2NL; BAG3; SETD8; TUBB; HSPA8; HSPA6; HSPA5; HSPA4; HSPA2; HSPA1L; DNAJA1; ETV6
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-HDAC6 (ARP31451_P050) antibody is Catalog # AAP31451 (Previous Catalog # AAPP02213)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HDAC6
• Uniprot ID: Q9UBN7
• Protein Name: HDAC6 protein EMBL AAH05872.1
• Sample Type Confirmation:

  There is BioGPS gene expression data showing that HDAC6 is expressed in HEK293T

• Protein Accession #: AAH05872
• Purification: Affinity Purified
• Nucleotide Accession #: NM_006044
• Tested Species Reactivity: Human
• Gene Symbol: HDAC6
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit
• Application: WB, CHIP, IHC
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 86%; Pig: 100%; Rabbit: 100%; Rat: 93%
• Image 1: Human 293T
  
  WB Suggested Anti-HDAC6 Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:62500
  Positive Control: 293T cell lysateThere is BioGPS gene expression data showing that HDAC6 is expressed in HEK293T
• Image 2: HCT116
  
  Chromatin Immunoprecipitation (ChIP) Using HDAC6 antibody - N-terminal region (ARP31451_P050) and HCT116 Cells
• Image 3: Human Kidney
  
  Rabbit Anti-HDAC6 Antibody
  Catalog Number: ARP31451_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Kidney Tissue
  Observed Staining: Cytoplasm
  Primary Antibody Concentration: 1:100
  Other Working Concentrations: N/A
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 - 2.0 sec
• Image 4: heart
  
  Rabbit Anti-HDAC6 Antibody
  Catalog Number: ARP31451_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Adult heart
  Observed Staining: Cytoplasmic,Nuclear
  Primary Antibody Concentration: 1:600
  Secondary Antibody: Donkey anti-Rabbit-Cy2/3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 – 2.0 sec
  Protocol located in Reviews and Data.
• Image 5:
  
  Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.