MXI1 Antibody - middle region (ARP31402_P050)

• Product Number: ARP31402_P050
• Name: MXI1 Antibody - middle region (ARP31402_P050)
• Protein Size (# AA): 295 amino acids
• Molecular Weight: 33kDa
• NCBI Gene Id: 4601
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: MAX interactor 1
• Alias Symbols: MXI, MAD2, MXD2, bHLHc11
• Peptide Sequence: Synthetic peptide located within the following region: QGPQEMERIRMDSIGSTISSDRSDSEREEIEVDVESTEFSHGEVDNISTT
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Johnson,K.R., (2007) FEBS J. 274 (17), 4643-4653
• Description of Target: Expression of the c-myc gene, which produces an oncogenic transcription factor, is tightly regulated in normal cells but is frequently deregulated in human cancers. The MXI1 gene encodes a transcriptional repressor protein thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in MXI1 are frequently found in patients with prostate tumors. Expression of the c-myc gene, which produces an oncogenic transcription factor, is tightly regulated in normal cells but is frequently deregulated in human cancers. The protein encoded by this gene is a transcriptional repressor thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in this gene are frequently found in patients with prostate tumors. Three alternatively spliced transcripts encoding different isoforms have been described. Additional alternatively spliced transcripts may exist but the products of these transcripts have not been verified experimentally.
• Protein Interactions: NOTCH2NL; KRTAP10-3; KRTAP10-8; KRTAP10-5; KRTAP10-9; KRTAP10-7; KRT40; RPL23AP32; CALCOCO2; MAX; ENTPD5; UBC; APP; CDC20; BUB1B; SIN3B; SIN3A; SMC3; MYC;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-MXI1 (ARP31402_P050) antibody is Catalog # AAP31402 (Previous Catalog # AAPP03065)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MXI1
• Uniprot ID: P50539-3
• Protein Name: Max-interacting protein 1
• Sample Type Confirmation:

  MXI1 is supported by BioGPS gene expression data to be expressed in SHSY5Y

• Protein Accession #: NP_569157
• Purification: Affinity Purified
• Nucleotide Accession #: NM_130439
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: MXI1
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 79%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 90%; Zebrafish: 77%
• Image 1: Mouse Testis
  
  Host: Mouse
  Target Name: MXI1
  Sample Tissue: Mouse Testis
  Antibody Dilution: 1ug/ml
• Image 2: Human SH-SYSY
  
  WB Suggested Anti-MXI1 Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:62500
  Positive Control: SH-SYSY cell lysateMXI1 is supported by BioGPS gene expression data to be expressed in SHSY5Y