Product Number |
ARP31380_P050 |
Product Page |
www.avivasysbio.com/nfya-antibody-c-terminal-region-arp31380-p050.html |
Name |
NFYA Antibody - C-terminal region (ARP31380_P050) |
Protein Size (# AA) |
347 amino acids |
Molecular Weight |
37kDa |
Subunit |
alpha |
NCBI Gene Id |
4800 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Nuclear transcription factor Y, alpha |
Alias Symbols |
HAP2, CBF-A, CBF-B, NF-YA |
Peptide Sequence |
Synthetic peptide located within the following region: YLHESRHRHAMARKRGEGGRFFSPKEKDSPHMQDPNQADEEAMTQIIRVS |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Zhu,Q.S., et al., (2004) J.Biol.Chem.279(29),29902-29910 |
Description of Target |
NFYA is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. |
Protein Interactions |
LUC7L2; POGZ; NFYB; GTF2A1; CSNK2A1; UBC; APPBP2; TP63; TP53; NFYC; EP300; CDK2; CCNA2; CDC25A; APP; RPS6KA6; JUN; HDAC1; ATF2; SP1; TAF6; TAF11; TAF12; PAPOLG; PWP1; SREBF2; ZHX2; ZHX1; ZHX3; KPNB1; SRSF1; HMGA1; SRF; USF2; USF1; NFYA; NR5A1; POU2F1; CEB |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-NFYA (ARP31380_P050) antibody |
Blocking Peptide |
For anti-NFYA (ARP31380_P050) antibody is Catalog # AAP31380 (Previous Catalog # AAPP02135) |
Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human NFYA |
Uniprot ID |
P23511 |
Protein Name |
Nuclear transcription factor Y subunit alpha |
Sample Type Confirmation |
NFYA is strongly supported by BioGPS gene expression data to be expressed in Jurkat |
Protein Accession # |
NP_002496 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_002505 |
Tested Species Reactivity |
Human |
Gene Symbol |
NFYA |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit |
Application |
IHC, WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100% |
Image 1 | Human Liver
| Human Liver |
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Image 2 | Human Muscle
| HumanMuscle |
|
Image 3 | Human Jurkat
| WB Suggested Anti-NFYA Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive Control: Jurkat cell lysateNFYA is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells |
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