| Product Number |
ARP31263_T100 |
| Product Page |
www.avivasysbio.com/nfya-antibody-c-terminal-region-arp31263-t100.html |
| Name |
NFYA Antibody - C-terminal region (ARP31263_T100) |
| Protein Size (# AA) |
347 amino acids |
| Molecular Weight |
37kDa |
| Subunit |
alpha |
| NCBI Gene Id |
4800 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1.0 mg/ml |
| Gene Full Name |
Nuclear transcription factor Y, alpha |
| Alias Symbols |
HAP2, CBF-A, CBF-B, NF-YA |
| Peptide Sequence |
Synthetic peptide located within the following region: YLHESRHRHAMARKRGEGGRFFSPKEKDSPHMQDPNQADEEAMTQIIRVS |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Zhu,Q.S.,et al.,(2004) J.Biol.Chem.279(29):29902-29910 |
| Description of Target |
NFYA is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. |
| Protein Interactions |
LUC7L2; POGZ; NFYB; GTF2A1; CSNK2A1; UBC; APPBP2; TP63; TP53; NFYC; EP300; CDK2; CCNA2; CDC25A; APP; RPS6KA6; JUN; HDAC1; ATF2; SP1; TAF6; TAF11; TAF12; PAPOLG; PWP1; SREBF2; ZHX2; ZHX1; ZHX3; KPNB1; SRSF1; HMGA1; SRF; USF2; USF1; NFYA; NR5A1; POU2F1; CEB |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-NFYA (ARP31263_T100) antibody |
| Blocking Peptide |
For anti-NFYA (ARP31263_T100) antibody is Catalog # AAP31263 (Previous Catalog # AAPP02012) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human NFYA |
| Uniprot ID |
P23511 |
| Protein Name |
Nuclear transcription factor Y subunit alpha |
| Sample Type Confirmation |
NFYA is strongly supported by BioGPS gene expression data to be expressed in Jurkat |
| Protein Accession # |
NP_002496 |
| Purification |
Protein A purified |
| Nucleotide Accession # |
NM_002505 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
NFYA |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Yeast |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 80% |
| Image 1 | Human Liver
 | Human Liver |
|
| Image 2 | Human Jurkat
 | WB Suggested Anti-NFYA Antibody Titration: 5.0-8.0ug/ml
ELISA Titer: 1:312500
Positive Control: Jurkat cell lysateNFYA is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells |
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