| Product Number |
ARP31200_P050 |
| Product Page |
www.avivasysbio.com/isgf3g-antibody-n-terminal-region-arp31200-p050.html |
| Name |
ISGF3G Antibody - N-terminal region (ARP31200_P050) |
| Protein Size (# AA) |
393 amino acids |
| Molecular Weight |
44 kDa |
| NCBI Gene Id |
10379 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Interferon regulatory factor 9 |
| Alias Symbols |
p48, IRF-9, ISGF3, ISGF3G |
| Peptide Sequence |
Synthetic peptide located within the following region: PWKHAGKQDFREDQDAAFFKAWAIFKGKYKEGDTGGPAVWKTRLRCALNK |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Lau,J.F., et al., (2003) Mol. Cell. Biol. 23 (2), 620-628 |
| Description of Target |
ISGF3G functions to recruit RNA polymerase II to the promoter of interferon-stimulated genes and requires histone deacetylases. Defects in ISGF3 can cause resistance to IFN-.(2a) treatment. |
| Protein Interactions |
S100A13; MYO1F; TOP1; IRF1; IRF2BP1; DNMT3A; ELAVL1; STAT2; IFNAR2; CREBBP; DACH2; DMRTC2; TLX3; VENTX; ARID3B; NR2E3; POU2F1; HOXC9; TLX2; STAT1; IFNA1; RELA; NFKB1; |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Enhanced Validation |
| Relative Expression (Western Blot) |
 |
| SPR Affinity Characterization |
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| Datasheets/Manuals |
Printable datasheet for anti-IRF9 (ARP31200_P050) antibody |
| Blocking Peptide |
For anti-IRF9 (ARP31200_P050) antibody is Catalog # AAP31200 (Previous Catalog # AAPS26406) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human ISGF3G |
| Uniprot ID |
Q00978 |
| Protein Name |
Interferon regulatory factor 9 |
| Publications |
Bidwell, B. N. et al. Silencing of Irf7 pathways in breast cancer cells promotes bone metastasis through immune escape. Nat. Med. 18, 1224-31 (2012). 22820642 |
| Protein Accession # |
NP_006075 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_006084 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
IRF9 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 79% |
| Image 1 | Human Liver
 | Human Liver |
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| Image 2 |
 | 25 ug of the indicated Human whole cell or tissue extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. A 35 kDa isoform contains the peptide sequence. |
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| Image 3 |
 | Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
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