| Product Number |
ARP30307_P050 |
| Product Page |
http://www.avivasysbio.com/anti-tp53-antibody-n-terminal-region-arp30307-p050.html |
| Product Name |
TP53 antibody - N-terminal region (ARP30307_P050) |
| Size |
50ug |
| Gene Symbol |
TP53 |
| Alias Symbols |
LFS1; TRP53; p53 |
| Nucleotide Accession# |
NM_000546 |
| Protein Accession# |
NP_000537 |
| Swissprot Id |
P04637 |
| Protein Size (# AA) |
393 amino acids |
| Molecular Weight |
44kDa |
| Product Format |
Lyophilized powder |
| NCBI Gene Id |
7157 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Purification |
Affinity Purified |
| Description |
This is a rabbit polyclonal antibody against TP53. It was validated on Western Blot using a cell lysate as a positive control. Aviva Systems Biology strives to provide antibodies covering each member of a whole protein family of your interest. We also use our best efforts to provide you antibodies recognize various epitopes of a target protein. For availability of antibody needed for your experiment, please inquire (info@avivasysbio.com). |
| Peptide Sequence |
Synthetic peptide located within the following region: EEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIE |
| Key Reference |
Boehme,K.A., (2008) Proc. Natl. Acad. Sci. U.S.A. 105 (22), 7785-7790 |
| Description of Target |
TP53 acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. |
| Partner Proteins |
BAK1, BCL6, APEX1, ATF3, ATM, ATM, ATM, ATM, ATR, AURKA, BAK1, BARD1, BAX, BAX, BAX, BCL2L1, BLM, BLM, BLM, BNIP3L, BRCA1, BRCA1, CARM1, CDC25C, CDKN1A, CDKN1A, CDKN1A, CDKN1A, CDKN1A, CR2, CREBBP, CUL9, DDX5, DHCR24, EP300, EP300, EP300, ERCC3, ESR1, FOXO3, GPS2, GSK3B, HCVgp1, HCVgp1, HCVgp1, HCVg |
| Reconstitution and Storage |
Add 50 μl of distilled water. Final anti-TP53 antibody concentration is 1 mg/ml in PBS buffer. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles. |
| Additional Information |
IHC Information: Skin
IHC Information: Kidney |
| Lead Time |
Domestic: within 24 hours delivery
International: 3-5 business days |
| Blocking Peptide |
For anti-TP53 antibody is Catalog # AAP30307 (Previous Catalog # AAPS08710) |
| Immunogen |
The immunogen for anti-TP53 antibody: synthetic peptide directed towards the N terminal of human TP53 |
| Complete computational species homology data |
TP53 antibody - N-terminal region (ARP30307_P050) |
| Tissue Tool |
Find tissues and cell lines supported to express TP53.  |
| Reviews and Data |
Computational species homology for TP53 antibody (ARP30307); Customer Reviews for TP53 Antibody (ARP30307_P050) tested with U20S, PLKO cells in Chromatin Immunoprecipitation; Product Protocols: TP53 antibody tested with Human 293T Cells (ARP30307_P050); P |
| Homology Short |
Bovine, Human, Horse |
| Observed Species Reactivity |
Human, Pig, Rabbit, Bovine |
| Application |
WB, IHC, ChIP |
| Predicted Homology Based on Immunogen Sequence |
Bovine: 100%; Human: 100%; Horse: 90% |
| Image 1 | Human 293T
 | WB Suggested Anti-TP53 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: 293T cell lysate |
| | Image 2 | Human U2OS, PLKO
 | Immunohistochemistry with U20S/ PLKO cells tissue |
| | Image 3 | Human U2OS, PLKO
 | Immunohistochemistry with U20S/ PLKO cells tissue |
| | Image 4 | Human U2OS, PLKO
 | Sample Type : U20S/ PLKO cells |
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