CHEK2 Antibody - N-terminal region (ARP30290_P050)

Data Sheet
 
Product Number ARP30290_P050
Product Page www.avivasysbio.com/chek2-antibody-n-terminal-region-arp30290-p050.html
Name CHEK2 Antibody - N-terminal region (ARP30290_P050)
Protein Size (# AA) 514 amino acids
Molecular Weight 58kDa
NCBI Gene Id 11200
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Checkpoint kinase 2
Alias Symbols CDS1, CHK2, LFS2, RAD53, hCds1, HuCds1, PP1425
Peptide Sequence Synthetic peptide located within the following region: MSRESDVEAQQSHGSSACSQPHGSVTQSQGSSSQSQGISSSSTSTMPNSS
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Kobayashi,A., (2006) Histol. Histopathol. 21 (4), 373-382
Description of Target In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. CHEK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, CHEK2 is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in its gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in its gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases.In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
Protein Interactions SRPK1; PPP2CA; PLK1; CHEK2; PRKDC; ATM; POLR2L; RCHY1; VHL; UBC; NR4A1; ELAVL1; CDC25A; ARHGAP1; PER3; CDC25C; CUL1; CUL4A; KPNA2; APP; STRAP; TP53; BRCA1; RB1; DAPK3; UBR5; PML; MDM2; BRCA2; KAT2B; LATS2; PSME3; TRIM28; RNF8; STAT1; PPP2R5E; PPP2R5D; PPP
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-CHEK2 (ARP30290_P050) antibody
Blocking Peptide For anti-CHEK2 (ARP30290_P050) antibody is Catalog # AAP30290 (Previous Catalog # AAPS08701)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human CHEK2
Uniprot ID O96017-12
Protein Name Serine/threonine-protein kinase Chk2
Sample Type Confirmation

CHEK2 is supported by BioGPS gene expression data to be expressed in Jurkat

Protein Accession # NP_665861
Purification Affinity Purified
Nucleotide Accession # NM_145862
Tested Species Reactivity Human
Gene Symbol CHEK2
Predicted Species Reactivity Human
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Human: 100%
Image 1
Human Jurkat
WB Suggested Anti-CHEK2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Jurkat cell lysateCHEK2 is supported by BioGPS gene expression data to be expressed in Jurkat
Image 2
Human MCF7 Whole Cell
Host: Rabbit
Target Name: CHEK2
Sample Tissue: Human MCF7 Whole Cell
Antibody Dilution: 1ug/ml
 

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