Product Number |
ARP30290_P050 |
Product Page |
www.avivasysbio.com/chek2-antibody-n-terminal-region-arp30290-p050.html |
Name |
CHEK2 Antibody - N-terminal region (ARP30290_P050) |
Protein Size (# AA) |
514 amino acids |
Molecular Weight |
58kDa |
NCBI Gene Id |
11200 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Checkpoint kinase 2 |
Alias Symbols |
CDS1, CHK2, LFS2, RAD53, hCds1, HuCds1, PP1425 |
Peptide Sequence |
Synthetic peptide located within the following region: MSRESDVEAQQSHGSSACSQPHGSVTQSQGSSSQSQGISSSSTSTMPNSS |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Kobayashi,A., (2006) Histol. Histopathol. 21 (4), 373-382 |
Description of Target |
In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. CHEK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, CHEK2 is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in its gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in its gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases.In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. |
Protein Interactions |
SRPK1; PPP2CA; PLK1; CHEK2; PRKDC; ATM; POLR2L; RCHY1; VHL; UBC; NR4A1; ELAVL1; CDC25A; ARHGAP1; PER3; CDC25C; CUL1; CUL4A; KPNA2; APP; STRAP; TP53; BRCA1; RB1; DAPK3; UBR5; PML; MDM2; BRCA2; KAT2B; LATS2; PSME3; TRIM28; RNF8; STAT1; PPP2R5E; PPP2R5D; PPP |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-CHEK2 (ARP30290_P050) antibody |
Blocking Peptide |
For anti-CHEK2 (ARP30290_P050) antibody is Catalog # AAP30290 (Previous Catalog # AAPS08701) |
Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human CHEK2 |
Uniprot ID |
O96017-12 |
Protein Name |
Serine/threonine-protein kinase Chk2 |
Sample Type Confirmation |
CHEK2 is supported by BioGPS gene expression data to be expressed in Jurkat |
Protein Accession # |
NP_665861 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_145862 |
Tested Species Reactivity |
Human |
Gene Symbol |
CHEK2 |
Predicted Species Reactivity |
Human |
Application |
IHC, WB |
Predicted Homology Based on Immunogen Sequence |
Human: 100% |
Image 1 | Human Jurkat
| WB Suggested Anti-CHEK2 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positive Control: Jurkat cell lysateCHEK2 is supported by BioGPS gene expression data to be expressed in Jurkat |
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Image 2 | Human MCF7 Whole Cell
| Host: Rabbit Target Name: CHEK2 Sample Tissue: Human MCF7 Whole Cell Antibody Dilution: 1ug/ml |
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