TRAF6 Antibody - middle region (ARP30226_P050)

• Product Number: ARP30226_P050
• Name: TRAF6 Antibody - middle region (ARP30226_P050)
• Protein Size (# AA): 522 amino acids
• Molecular Weight: 59kDa
• NCBI Gene Id: 7189
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: TNF receptor-associated factor 6, E3 ubiquitin protein ligase
• Alias Symbols: RNF85, MGC:3310
• Peptide Sequence: Synthetic peptide located within the following region: YVTFMHLEALRQRTFIKDDTLLVRCEVSTRFDMGSLRREGFQPRSTDAGV
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Jazdzewski,K., (2008) Proc. Natl. Acad. Sci. U.S.A. 105 (20), 7269-7274
• Description of Target: TRAF6 is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. This protein mediates the signaling not only from the members of the TNF receptor superfamily, but also from the members of the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/RANCE and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase (IKK) in response to proinflammatory cytokines. The interaction of this protein with UBE2N/UBC13, and UBE2V1/UEV1A, which are ubiquitin conjugating enzymes catalyzing the formation of polyubiquitin chains, has been found to be required for IKK activation by this protein.The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. This protein mediates the signaling not only from the members of the TNF receptor superfamily, but also from the members of the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/RANCE and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase (IKK) in response to proinflammatory cytokines. The interaction of this protein with UBE2N/UBC13, and UBE2V1/UEV1A, which are ubiquitin conjugating enzymes catalyzing the formation of polyubiquitin chains, has been found to be required for IKK activation by this protein. Two alternatively spliced transcript variants encoding identical proteins have been reported.
• Protein Interactions: YES1; UBE2V1; UBE2N; UBE2L3; UBE2E1; UBE2D2; UBE2D1; POLI; UBC; IL1R1; EDARADD; PLEKHF2; LAT; TRAF3IP2; PPP4R1; USP2; IKBKG; TRAF6; TRAF5; TRAF2; TRAF1; CNTN2; SYK; VPS52; PSEN1; LGMN; MEOX2; MCL1; CANX; nef; TAB2; OTUD7A; GTF2I; KRAS; DCBLD2; MAP3K7; EIF
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-TRAF6 (ARP30226_P050) antibody is Catalog # AAP30226 (Previous Catalog # AAPS09104)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TRAF6
• Uniprot ID: Q9Y4K3
• Protein Name: TNF receptor-associated factor 6
• Protein Accession #: NP_004611
• Purification: Affinity Purified
• Nucleotide Accession #: NM_004620
• Tested Species Reactivity: Human
• Gene Symbol: TRAF6
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Horse, Pig, Rabbit
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%
• Image 1: Human 293T
  
  WB Suggested Anti-TRAF6 Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:312500
  Positive Control: 293T cell lysate
• Image 2: Human Fetal Lung
  
  Host: Rabbit
  Target Name: TRAF6
  Sample Type: Human Fetal Lung
  Antibody Dilution: 1.0ug/ml
• Image 3: Human Fetal Liver
  
  Host: Rabbit
  Target Name: TRAF6
  Sample Type: Human Fetal Liver
  Antibody Dilution: 1.0ug/ml
• Image 4: Human Adult Placenta
  
  Host: Rabbit
  Target Name: TRAF6
  Sample Type: Human Adult Placenta
  Antibody Dilution: 1.0ug/ml