Product Number |
ARP30161_P050 |
Product Page |
www.avivasysbio.com/ccnb1-antibody-c-terminal-region-arp30161-p050.html |
Name |
CCNB1 Antibody - C-terminal region (ARP30161_P050) |
Protein Size (# AA) |
433 amino acids |
Molecular Weight |
48kDa |
NCBI Gene Id |
891 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Cyclin B1 |
Alias Symbols |
CCNB |
Peptide Sequence |
Synthetic peptide located within the following region: YTEESLLPVMQHLAKNVVMVNQGLTKHMTVKNKYATSKHAKISTLPQLNS |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Zhao,M., (2006) Exp Oncol 28 (1), 44-48 |
Description of Target |
CCNB1 is a regulatory protein involved in mitosis. CCNB1 complexes with p34(cdc2) to form the maturation-promoting factor (MPF).The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. |
Protein Interactions |
CDC27; CDC20; CDH1; ANAPC11; FZR1; ANAPC4; SIRT1; BTRC; CDC16; UBC; CDKN1A; VCP; Samhd1; CKS2; CKS1B; RHOBTB3; CDK1; CDK5; CDK2; SQSTM1; PTMA; TP73; TP53BP1; POLA1; RB1; CDK7; HIST1H1B; CDC6; CCNB1IP1; MFN1; MARCH5; PRC1; HIST1H1A; SDHAF2; NDC80; CDT1; RP |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-CCNB1 (ARP30161_P050) antibody |
Other Applications Image 1 Data |
IP Suggested Anti-CCNB1 Antibody Positive Control: NT2 CELL/BRAIN TISSUE
|
Other Applications Image 2 Data |
IP Suggested Anti-CCNB1 antibody Titration: 2 ug/ml Positive Control: Mouse brain homogenate
|
Blocking Peptide |
For anti-CCNB1 (ARP30161_P050) antibody is Catalog # AAP30161 (Previous Catalog # AAPS08606) |
Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human CCNB1 |
Uniprot ID |
P14635 |
Protein Name |
G2/mitotic-specific cyclin-B1 |
Sample Type Confirmation |
CCNB1 is supported by BioGPS gene expression data to be expressed in Jurkat |
Protein Accession # |
NP_114172 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_031966 |
Tested Species Reactivity |
Human, Mouse |
Gene Symbol |
CCNB1 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit |
Application |
IP, WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 92%; Dog: 85%; Guinea Pig: 92%; Horse: 85%; Human: 100%; Mouse: 92%; Rabbit: 92%; Rat: 92% |
Image 1 | Human Lymph Node
| Rabbit Anti-CCNB1 Antibody Catalog Number: ARP30161_P050 Formalin Fixed Paraffin Embedded Tissue: Human Lymph Node Tissue Observed Staining: Cytoplasm Primary Antibody Concentration: 1:600 Other Working Concentrations: N/A Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec |
|
Image 2 | Mouse Brain
| Researcher: Dr. Yuzhi Chen, University of Arkansas for Medical Science Application: IP Species+tissue/cell type: Mouse brain homogenate How many ug'sof tissue/cell lysate run on the gel: 500 ug mouse brain homogenateIP antibody: 6 ug Primary antibody dilution: 1:500 Secondary antibody: Goat anti-rabbit Alexa-Fluor 594 Secondary antibody dilution: 1:5000 |
|
Image 3 | Human NT2
| Researcher:Dr. Yuzhi Chen, University of Arkansas for Medical Science Application:Western blotting Species+tissue/cell type: lane 1: 60ug human NT2 cell line Primary antibody dilution: 1:500 Secondary antibody:IRDye 800 CW goat anti-rabbit from Li-COR Bioscience Secondary antibody dilution:1:20,000 |
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Image 4 | human NT2 line
| WB Suggested Anti-CCNB1 Antibody Positive Control: Lane 1: 60ug human NT2 cell line Primary Antibody Dilution : 1:500 Secondary Antibody : IRDye 800 CW goat anti-rabbit from Li-COR Bioscience Secondry Antibody Dilution : 1:20,000 Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science |
|
Image 5 | Human Jurkat
| WB Suggested Anti-CCNB1 Antibody Titration: 0.125ug/ml ELISA Titer: 1:312500 Positive Control: Jurkat cell lysateCCNB1 is supported by BioGPS gene expression data to be expressed in Jurkat |
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