TAF15 Antibody - N-terminal region (ARP30111_T100)

• Product Number: ARP30111_T100
• Name: TAF15 Antibody - N-terminal region (ARP30111_T100)
• Protein Size (# AA): 592 amino acids
• Molecular Weight: 62kDa
• NCBI Gene Id: 8148
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa
• Alias Symbols: Npl3, RBP56, TAF2N, TAFII68
• Peptide Sequence: Synthetic peptide located within the following region: GGRGRGGYDKDGRGPMTGSSGGDRGGFKNFGGHRDYGPRTDADSESDNSD
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Lee,H.J., et al., (2004) FEBS Lett. 564 (1-2), 188-198
• Description of Target: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. TAF15 encodes a subunit of TFIID present in a subset of TFIID complexes. Translocations involving chromosome 17 and chromosome 9, where the gene for the nuclear receptor CSMF is located, result in a gene fusion product that is an RNA binding protein associated with a subset of extraskeletal myxoid chondrosarcomas.
• Protein Interactions: RPA3; RPA2; RPA1; SUZ12; RNF2; EZH2; BMI1; EED; UBD; PRMT8; MED26; UBC; FUS; TPR; TPM2; DDB1; SCARB2; RNF168; TMED9; SAP30BP; NEDD8; CAND1; DCUN1D1; COPS5; COPS6; CUL1; CUL2; CUL3; CUL4A; CUL4B; CUL5; YWHAZ; SIRT7; HIST2H4A; HIST1H3A; HIST2H2AC; CACNA1A;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-TAF15 (ARP30111_T100) antibody is Catalog # AAP30111 (Previous Catalog # AAPH00287)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TAF15
• Uniprot ID: Q92804
• Protein Name: TATA-binding protein-associated factor 2N
• Publications:

  Perez, K. J., Martínez, F. P., Cosme-Cruz, R., Perez-Crespo, N. M. & Tang, Q. A short cis-acting motif in the M112-113 promoter region is essential for IE3 to activate M112-113 gene expression and is important for murine cytomegalovirus replication. J. Virol. 87, 2639-47 (2013). 23255797

• Protein Accession #: NP_631961
• Purification: Protein A purified
• Nucleotide Accession #: NM_139215
• Tested Species Reactivity: Human
• Gene Symbol: TAF15
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 93%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 93%; Rabbit: 93%; Rat: 93%
• Image 1: Human HepG2
  
  WB Suggested Anti-TAF15 Antibody Titration: 3.0ug/ml
  ELISA Titer: 1:312500
  Positive Control: HepG2 cell lysate