RBM10 Antibody - N-terminal region (ARP30104_T100)

• Product Number: ARP30104_T100
• Name: RBM10 Antibody - N-terminal region (ARP30104_T100)
• Protein Size (# AA): 930 amino acids
• Molecular Weight: 104 kDa
• NCBI Gene Id: 8241
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1.0 mg/ml
• Gene Full Name: RNA binding motif protein 10
• Alias Symbols: S1-1, TARPS, GPATC9, ZRANB5, GPATCH9, DXS8237E
• Peptide Sequence: Synthetic peptide located within the following region: QRRRRRRHRHSPTGPPGFPRDGDYRDQDYRTEQGEEEEEEEDEEEEEKAS
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Thiselton,D.L., et al., (2002) Genomics 79 (4), 560-572
• Description of Target: RBM10 contains RNA recognition motif found in a variety of RNA binding proteins, including various hnRNP proteins, proteins implicated in regulation of alternative splicing, and protein components of snRNPs. In vitro studies showed that the rat homolog bound to RNA homopolymers, with a preference for G and U polyribonucleotides. This gene is part of a gene cluster on chromosome Xp11.23, and its 3' end lies within 20 kb upstream of UBE1.
• Protein Interactions: TBC1D22B; AES; SUMO2; UBC; RPA3; RPA2; RPA1; ASB12; ZBTB1; FBXO6; PTBP2; LMNA; PAN2; SUGP1; CCAR1; PRPF19; GPKOW; U2SURP; U2AF2; SF3A1; SF3B4; DHX38; RBM10; SF1; WHSC1; U2AF1; SNRPC; SRSF6; RBM4; PTBP1; PRCC; IK; HNRNPK; HNRNPH2; DHX15; DDX5; CSNK2A1; SMU
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation:

  WB	Y
  SPR
  YCHAROS

• Blocking Peptide: For anti-RBM10 (ARP30104_T100) antibody is Catalog # AAP30104 (Previous Catalog # AAPH00280)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RBM10
• Uniprot ID: P98175
• Protein Name: RNA-binding protein 10
• Sample Type Confirmation:

  RBM10 is strongly supported by BioGPS gene expression data to be expressed in Daudi

• Protein Accession #: NP_005667
• Purification: Protein A purified
• Nucleotide Accession #: NM_005676
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: RBM10
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Application: IHC, WB, IP
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 92%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Image 1: Human Lung
  
  Rabbit Anti-RBM10 Antibody
  Catalog Number: ARP30104
  Paraffin Embedded Tissue: Human Lung
  Cellular Data: Epithelial cells of bronchiole
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X
• Image 2: Human Heart
  
  Rabbit Anti-RBM10 Antibody
  Catalog Number: ARP30104
  Paraffin Embedded Tissue: Human Heart
  Cellular Data: Myocardial cells
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X
• Image 3: Human Daudi
  
  WB Suggested Anti-RBM10 Antibody Titration: 1.4ug/ml
  ELISA Titer: 1:1562500
  Positive Control: Daudi cell lysateRBM10 is strongly supported by BioGPS gene expression data to be expressed in Human Daudi cells
• Image 4: Human Heart
  
  Rabbit Anti-RBM10 Antibody
  Catalog Number: ARP30104
  Paraffin Embedded Tissue: Human cardiac cell
  Cellular Data: Epithelial cells of renal tubule
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X
• Image 5: HEK293 Whole Cell Lysate
  
  RBM10 was immunoprecipitated from 1 mg HEK293 Whole Cell Lysate with ARP30104_T100 with 1:200 dilution. Western blot was performed using ARP30104_T100 at 1/1000 dilution.
  Lane 1: Control IP in HEK293 Whole Cell Lysate.
  Lane 2: RBM10 IP with ARP30104_T100 in HEK293 Whole Cell Lysate.
  Lane 3: Input of HEK293 Whole Cell Lysate.
• Image 6: Mouse Liver
  
  Host: Mouse
  Target Name: RBM10
  Sample Tissue: Mouse Liver
  Antibody Dilution: 1ug/ml