C20ORF100 Antibody - N-terminal region (ARP30008_P050)

• Product Number: ARP30008_P050
• Name: C20ORF100 Antibody - N-terminal region (ARP30008_P050)
• Protein Size (# AA): 464 amino acids
• Molecular Weight: 49kDa
• NCBI Gene Id: 84969
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: TOX high mobility group box family member 2
• Alias Symbols: GCX1, GCX-1, C20orf100, dJ495O3.1, dJ1108D11.2
• Peptide Sequence: Synthetic peptide located within the following region: TSQTYNGQSENNEDYEIPPITPPNLPEPSLLHLGDHEASYHSLCHGLTPN
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Ota,T., et al., (2004) Nat. Genet. 36 (1), 40-45
• Description of Target: This gene, which is located on chromosome 20, is predicted to encode for a signal protein with unknown function.
• Protein Interactions: APP; UBC; ZNF461;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-TOX2 (ARP30008_P050) antibody is Catalog # AAP30008 (Previous Catalog # AAPH00108)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human C20ORF100
• Uniprot ID: Q96NM4
• Protein Name: TOX high mobility group box family member 2
• Protein Accession #: NP_116272
• Purification: Affinity Purified
• Nucleotide Accession #: NM_032883
• Tested Species Reactivity: Human
• Gene Symbol: TOX2
• Predicted Species Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Pig, Rabbit
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 93%; Rat: 100%
• Image 1: Human Intestine
  
  Rabbit Anti-C20orf100 Antibody
  Catalog Number: ARP30008
  Paraffin Embedded Tissue: Human Intestine
  Cellular Data: Epithelial cells of intestinal villas
  Antibody Concentration: 4.0-8.0 ug/ml
  Magnification: 400X
• Image 2: Human Jurkat
  
  WB Suggested Anti-C20ORF100 Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:312500
  Positive Control: Jurkat cell lysate
• Image 3:
  
  Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.