5-Methylcytosine Antibody (AMM99021)

Data Sheet
 
Product Number AMM99021
Product Page www.avivasysbio.com/5-methylcytosine-antibody-amm99021.html
Name 5-Methylcytosine Antibody (AMM99021)
Clone 33D3
Isotype IgG1
Host Mouse
Clonality Monoclonal
Purity > 95% (Gel electrophoresis SDS PAGE. Densitometry analysis)
Concentration 1 mg/ml
Description
Product Format Liquid.PBS pH 7.4 (NaCl 137 mM, KCI 2.7 mM, 10 mM Na2HPO4, 2 mM KH2PO4). No preservative.
Reference Maki W. C., et al., Biosens Bioelectron 23(6):780-787 (2008).
Walsh, T. K., et al., Insect Mol Biol. 19 Suppl 2:215-28 (2010).
Brena, R. M., et al., Methods in Molecular Biology, 507, Part IV, 257-269 (2009).
Zhang, W., et al., Plant Cell. 20(1):25-34 (2008).
Zhang, W., et al, Genome Res. 18(12):1938-1943 (2008).
Jensen, T. J., et al., Carcinogenesis.29(8):1500-1508 (2008).
Maki W. C., et al., Biosens Bioelectron 23(6):780-787 (2008).
Vrba, L., et al., BMC Genomics. 15;9:486 (2008).
Vinsky, M. D., et al., Reprod Fertil Dev. 19(5):603-611 (2007).
Novak, P., et al., Cancer Res. 66(22):10664-10670 (2006).
Description of Target Methylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 60 to 90% of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure.
Reconstitution and Storage Ready for usage. For longer periods of storage, store at -20C. Can be stored 1 month at 4C.Avoid repeat freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for AMM99021
Additional Information Sterility Test: 0 CFU (on blood agar).
Intended Use For research use only.
Publications

Conformation-selective methylation of geminivirus DNA. J Virol. 85, 12001-12 (2011) 21835804

Dynamic heterogeneity of DNA methylation and hydroxymethylation in embryonic stem cell populations captured by single-cell 3D high-content analysis. Exp Cell Res. 332, 190-201 (2015). 25700729

Fukuda, K. et al. Regional DNA methylation differences between humans and chimpanzees are associated with genetic changes, transcriptional divergence and disease genes. J. Hum. Genet. 58, 446-54 (2013). 23739127

Gertych, A., Oh, J. H., Wawrowsky, K. A., Weisenberger, D. J. & Tajbakhsh, J. 3-D DNA methylation phenotypes correlate with cytotoxicity levels in prostate and liver cancer cell models. BMC Pharmacol. Toxicol. 14, 11 (2013). 23394161

Global acetylation and methylation changes predict papillary urothelial neoplasia of low malignant potential recurrence: a quantitative analysis. Int J Immunopathol Pharmacol. 24, 489-97 (2011) 21658323

Jensen, T. J., Novak, P., Eblin, K. E., Gandolfi, A. J. & Futscher, B. W. Epigenetic remodeling during arsenical-induced malignant transformation. Carcinogenesis 29, 1500-8 (2008). 18448484

Kurita, R. & Niwa, O. DNA methylation analysis triggered by bulge specific immuno-recognition. Anal. Chem. 84, 7533-8 (2012). 22880797

Kurita, R., Arai, K., Nakamoto, K., Kato, D. & Niwa, O. Determination of DNA methylation using electrochemiluminescence with surface accumulable coreactant. Anal. Chem. 84, 1799-803 (2012). 22263690

Macaulay, E. C., Weeks, R. J., Andrews, S. & Morison, I. M. Hypomethylation of functional retrotransposon-derived genes in the human placenta. Mamm. Genome 22, 722-35 (2011). 21874386

Maki, W. C. et al. Nanowire-transistor based ultra-sensitive DNA methylation detection. Biosens. Bioelectron. 23, 780-7 (2008). 17936611

Microplate-based platform for combined chromatin and DNA methylation immunoprecipitation assays. BMC Mol Biol. 12, 49 (2011). 22098709

Minardi, D. et al. Prognostic role of global DNA-methylation and histone acetylation in pT1a clear cell renal carcinoma in partial nephrectomy specimens. J. Cell. Mol. Med. 13, 2115-21 (2009). 18752633

Physical localization and DNA methylation of 45S rRNA gene loci in Jatropha curcas L. PLoS One. 8, e84284 (2014). 24386362

PRDM14 is expressed in germ cell tumors with constitutive overexpression altering human germline differentiation and proliferation. Stem Cell Res. 27, 46-56 (2018). 29324254

Primate Primordial Germ Cells Acquire Transplantation Potential by Carnegie Stage 23. Stem Cell Reports. 9, 329-341 (2017). 28579394

Prostate cancer diagnosis using epigenetic biomarkers, 3D high-content imaging and probabilistic cell-by-cell classifiers. Oncotarget. 8, 57278-57301 (2017). 28915670

Ribas, R. et al. Members of the TEAD family of transcription factors regulate the expression of Myf5 in ventral somitic compartments. Dev. Biol. 355, 372-80 (2011). 21497756

Single cell epigenetic visualization assay. Nucleic Acids Res. (2021). 33511400

Siyuan Chang, et al. Epigenetic regulation of osteopontin splicing isoform c defines its role as a microenvironmental factor to promote the survival of colon cancer cells from 5-FU treatment. 452 (2020). 32944000

Tajbakhsh, J. Covisualization of methylcytosine, global DNA, and protein biomarkers for In Situ 3D DNA methylation phenotyping of stem cells. Methods Mol. Biol. 1052, 77-88 (2013). 23592032

The Sm protein methyltransferase PRMT5 is not required for primordial germ cell specification in mice. EMBO J. 34, 748-58 (2015). 25519955

Utility of 5-Methylcytosine Immunohistochemical Staining to Assess Global DNA Methylation and Its Prognostic Impact in MDS Patients. Asian Pac J Cancer Prev. 18, 3307-3313 (2017). 29286224

Vrba, L., Junk, D. J., Novak, P. & Futscher, B. W. p53 induces distinct epigenetic states at its direct target promoters. BMC Genomics 9, 486 (2008). 18922183

Walsh, T. K. et al. A functional DNA methylation system in the pea aphid, Acyrthosiphon pisum. Insect Mol. Biol. 19 Suppl 2, 215-28 (2010). 20482652

Zhang, W., Lee, H.-R., Koo, D.-H. & Jiang, J. Epigenetic modification of centromeric chromatin: hypomethylation of DNA sequences in the CENH3-associated chromatin in Arabidopsis thaliana and maize. Plant Cell 20, 25-34 (2008). 18239133

Zhang, W., Wang, X., Yu, Q., Ming, R. & Jiang, J. DNA methylation and heterochromatinization in the male-specific region of the primitive Y chromosome of papaya. Genome Res. 18, 1938-43 (2008). 18593814

Purification Protein A ceramic hyper DF affinity chromatography
Predicted Species Reactivity Human, Mouse, Rat, Zebrafish, Dog, Pig, Chicken
Application FC, IHC-FP, ICC, IP, CHIP, RIA, IF
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