Catalog No: OKEH01867
Size:96T
Price: $625.00
SKU
OKEH01867
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
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- $55: Antibody & Protein in US
- $55 + $25/Kit in US
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SCUBE1 ELISA Kit (Human) (OKEH01867)
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology SCUBE1 ELISA Kit (Human) (OKEH01867) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SCUBE1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SCUBE1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SCUBE1 captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 110% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: < 6.5% (n = 20) Mean Inter-assay CV%: < 9.5% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
Sensitivity | 0.056 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human Signal peptide, CUB and EGF-like domain-containing protein 1 | ||||||||||||||||||||||
Publications | SCUBE1 Controls BMPR2-Relevant Pulmonary Endothelial Function: Implications for Diagnostic Marker Development in Pulmonary Arterial Hypertension. JACC Basic Transl Sci. 5, 1073-1092 (2020). 33294740 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||||||||
Description |
Gene Symbol | SCUBE1 |
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Gene Full Name | signal peptide, CUB domain and EGF like domain containing 1 |
Alias Symbols | signal peptide, CUB and EGF-like domain-containing protein 1;signal peptide, CUB domain, EGF-like 1;signal peptide-CUB domain-EGF-related 1. |
NCBI Gene Id | 80274 |
Protein Name | Q8IWY4 |
Description of Target | Signal peptide, CUB and EGF-like domain-containing protein 1 |
Uniprot ID | https://www.uniprot.org/uniprot/Q8IWY4 |
Protein Accession # | NP_766638.2 |
Nucleotide Accession # | NM_173050.3 |
Protein Size (# AA) | 988 |
Molecular Weight | 107 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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