Catalog No: OKCD02872
Size:96WELLS
Price: $650.00
SKU
OKCD02872
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Datasheets/ManualsPrintable datasheet for SCT ELISA Kit (Mouse) (OKCD02872)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration2h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)93-102%89-97%79-94%79-102%
EDTA Plasma (n=5)79-92%93-101%81-89%83-104%
Heparin Plasma (n=5)96-105%81-93%83-99%93-101%
ELISA Kit PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Secretin (SCT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Secretin (SCT) and unlabeled Secretin (SCT) (Standards or samples) with the pre-coated antibody specific to Secretin (SCT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Secretin (SCT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Secretin (SCT) in the sample.
ELISA Kit Range12.35-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)96-103101
EDTA Plasma (n=5)96-105101
Heparin Plasma (n=5)78-9689
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secretin (SCT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secretin (SCT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Sct Microplate96 Wells (12 x 8 Well strips)
Sct Lyophilized Standard 2 x 1 ng
100X Sct Biotin Complex1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 4.83 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Secretin (SCT). No significant cross-reactivity or interference between Secretin (SCT) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Competitive Inhibition ELISA
Gene SymbolSct
Gene Full Namesecretin
Alias Symbolssecretin.
NCBI Gene Id20287
Protein NameSecretin
Description of TargetStimulates formation of NaHCO(3)-rich pancreatic juice and secretion of NaHCO(3)-rich bile and inhibits HCl production by the stomach.
Uniprot IDQ08535
Protein Accession #NP_035458.1
Nucleotide Accession #NM_011328.3
Molecular Weight133 kDa
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