SCNN1A ELISA Kit (Human) (OKCD00380)

Catalog No: OKCD00380 (Formerly GWB-KBBJZ4)

Size:96WELLS

Human SCNN1A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	89-97%	87-101%	78-102%	79-95%
  EDTA plasma(n=5)	89-98%	78-102%	83-101%	78-102%
  heparin plasma(n=5)	86-96%	78-92%	87-102%	78-104%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	80-89	83
  EDTA plasma(n=5)	88-96	92
  heparin plasma(n=5)	81-103	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-SCNN1A Microplate: 96 Wells (12 x 8 Well Strips)
  • SCNN1A Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated SCNN1A Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.061 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a). No significant cross-reactivity or interference between Amiloride Sensitive Sodium Channel Subunit Alpha (SCNN1a) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: SCNN1A
• Gene Full Name: sodium channel epithelial 1 subunit alpha
• Alias Symbols: alpha ENaC-2;alpha-ENaC;alpha-NaCH;amiloride-sensitive epithelial sodium channel alpha subunit;amiloride-sensitive sodium channel subunit alpha;amiloride-sensitive sodium channel subunit alpha 2;BESC2;ENaCa;ENaCalpha;epithelial Na(+) channel subunit alpha;LIDLS3;nasal epithelial sodium channel alpha subunit;nonvoltage-gated sodium channel 1 subunit alpha;SCNEA;SCNN1;sodium channel epithelial 1 alpha subunit;sodium channel, non voltage gated 1 alpha subunit;sodium channel, nonvoltage-gated 1 alpha;sodium channel, non-voltage-gated 1 alpha.
• NCBI Gene Id: 6337
• Protein Name: Amiloride-sensitive sodium channel subunit alpha
• Description of Target: Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and eccrine sweat glands. Also plays a role in taste perception.
• Uniprot ID: P37088
• Protein Accession #: NP_001029
• Nucleotide Accession #: NM_001038.5
• Protein Size (# AA): 669