Catalog No: OKCD00205 (Formerly GWB-KBBJG1)
Size:96WELLS
Price: $700.00
SKU
OKCD00205
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Datasheets/ManualsPrintable datasheet for SCGN ELISA Kit (Mouse) (OKCD00205)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)83-90%99-105%90-104%80-89%
EDTA plasma(n=5)80-102%82-96%97-105%84-91%
heparin plasma(n=5)91-99%81-98%79-90%78-89%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secretagogin (SCGN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secretagogin (SCGN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secretagogin (SCGN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Secretagogin (SCGN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)78-104101
EDTA plasma(n=5)88-9793
heparin plasma(n=5)81-9487
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secretagogin (SCGN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secretagogin (SCGN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Scgn Microplate96 Wells (12 x 8 Well Strips)
Scgn Lyophilized Standard2 x 1 ng
100X Biotinylated Scgn Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 6.4 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Secretagogin (SCGN). No significant cross-reactivity or interference between Secretagogin (SCGN) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolScgn
Gene Full Namesecretagogin, EF-hand calcium binding protein
Alias Symbolssecretagogin.
NCBI Gene Id214189
Protein NameSecretagogin
Description of TargetSecretagogin
Uniprot IDQ91WD9
Protein Accession #NP_663374
Nucleotide Accession #NM_145399.1
Protein Size (# AA)276
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