SCG2 ELISA Kit (Rat) (OKCD02329)

Catalog No: OKCD02329

Size:96WELLS

Rat SCG2 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	78-104%	79-96%	81-96%	97-105%
  EDTA Plasma (n=5)	83-99%	79-90%	94-103%	91-98%
  Heparin Plasma (n=5)	79-98%	91-101%	78-89%	78-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chromogranin B (CHGB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chromogranin B (CHGB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chromogranin B (CHGB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Chromogranin B (CHGB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	95-104	101
  EDTA Plasma (n=5)	92-99	96
  Heparin Plasma(n=5)	97-105	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chromogranin B (CHGB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chromogranin B (CHGB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-SCG2 Microplate: 96 Wells (12 x 8 Well strips)
  • SCG2 Lyophilized Standard: 2
  • 100X Biotinylated SCG2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 0.129 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Chromogranin B (CHGB). No significant cross-reactivity or interference between Chromogranin B (CHGB) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Chgb
• Gene Full Name: chromogranin B
• Alias Symbols: cgB;chromogranin B (secretogranin 1);Chromogranin B, parathyroid secretory protein;Chromogranin-B;glucagonoma peptide;secretogranin-1;secretogranin-I;sgI.
• NCBI Gene Id: 24259
• Protein Name: O35314
• Description of Target: Secretogranin-1
• Uniprot ID: https://www.uniprot.org/uniprot/O35314
• Protein Accession #: NP_073160.1
• Nucleotide Accession #: NM_022669.1
• Protein Size (# AA): 619