Catalog No: OKCD02871
Size:96WELLS
Price: $675.00
SKU
OKCD02871
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Datasheets/ManualsPrintable datasheet for SCG2 ELISA Kit (Human) (OKCD02871)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)80-101%87-96%88-102%91-98%
EDTA Plasma (n=5)95-105%80-94%84-92%95-103%
Heparin Plasma (n=5)99-105%78-101%78-92%95-102%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secretogranin II (SCG2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secretogranin II (SCG2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secretogranin II (SCG2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Secretogranin II (SCG2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.25-80ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)98-105102
EDTA Plasma (n=5)84-9893
Heparin Plasma(n=5)90-9893
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secretogranin II (SCG2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secretogranin II (SCG2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-SCG2 Microplate96 Wells (12 x 8 Well strips)
SCG2 Lyophilized Standard2
100X Biotinylated SCG2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity0.49 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Secretogranin II (SCG2). No significant cross-reactivity or interference between Secretogranin II (SCG2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolSCG2
Gene Full Namesecretogranin II
Alias SymbolsCHGC;chromogranin-C;EM66;Secretogranin II;secretogranin-2;secretoneurin;SgII;SN.
NCBI Gene Id7857
Protein NameSecretogranin-2
Description of TargetSecretogranin-2 is a neuroendocrine secretory granule protein, which is the precursor for biologically active peptides.
Uniprot IDP13521
Protein Accession #NP_003460.2
Nucleotide Accession #NM_003469.4
Protein Size (# AA)617
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