Catalog No: OKCA01818
Size:96 Wells
Price: $857.00
SKU
OKCA01818
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRabbit
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean Recovery (%)Range (%)
ELISA Kit PrincipleAviva Systems Biology SAA2 ELISA Kit (Rabbit) (OKCA01818) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SAA2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SAA2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample SAA2 captured in the well.
ELISA Kit Range31.25 - 2,000 ng/mL
ELISA Kit Recovery
SampleMean Recovery (%)Range (%)
Serum (n=5)10195-107
EDTA Plasma (n=4)9284-97
ELISA Kit Component
ComponentAmount
SAA2 Microplate96 Wells (12 x 8 Well strips)
SAA2 Lyophilized Standard2 x 2 ug
100X Biotinylated SAA2 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity7.8 ng/mL
Assay InfoAssay Methodology: Quantitative Sanadwich ELISA
Protocol Information
Gene SymbolSAA2
Gene Full NameSerum amyloid A-2 protein
Alias SymbolsSAA2
NCBI Gene Id100009259
Protein NameSerum amyloid A-2 protein
Description of TargetMajor acute phase reactant. Apolipoprotein of the HDL complex.
Uniprot IDP22000
Protein Accession #NP_001075861.1
Nucleotide Accession #NM_001082392.1
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