S1PR5 ELISA Kit (Rat) (OKEH03090)

Catalog No: OKEH03090

Size:96T

Rat S1PR5 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology S1PR5 ELISA Kit (Rat) (OKEH03090) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for S1pr5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for S1pr5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample S1pr5 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Component:
  • Anti-S1pr5 Microplate: 96 Wells (12 x 8 Well strips)
  • S1pr5 Lyophilized Standard: 2
  • 100X Biotinylated S1pr5 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.091 ng/mL
• Specificity: Natural and recombinant Rat Sphingosine 1-phosphate receptor 5
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: S1pr5
• Gene Full Name: sphingosine-1-phosphate receptor 5
• Alias Symbols: Edg8;Edg-8;endothelial differentiation G-protein-coupled receptor 8;endothelial differentiation, sphingolipid G-protein-coupled receptor, 8;nerve growth factor-regulated G-protein-coupled receptor 1;NRG-1;S1P receptor 5;S1P receptor Edg-8;S1P5;sphingosine 1-phosphate receptor 5;sphingosine 1-phosphate receptor Edg-8.
• NCBI Gene Id: 60399
• Protein Name: Q9JKM5
• Description of Target: Sphingosine 1-phosphate receptor 5
• Uniprot ID: https://www.uniprot.org/uniprot/Q9JKM5
• Protein Accession #: NP_068543.2
• Nucleotide Accession #: NM_021775.2
• Protein Size (# AA): 400
• Molecular Weight: 42 kDa