Catalog No: OKCD00290 (Formerly GWB-KBBHR8)
Size:96WELLS
Price: $550.00
SKU
OKCD00290
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
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Datasheets/Manuals | Printable datasheet for S100A6 ELISA Kit (Mouse) (OKCD00290) |
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Predicted Species Reactivity | Mouse|Mus musculus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein A6 (S100A6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S100 Calcium Binding Protein A6 (S100A6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100 Calcium Binding Protein A6 (S100A6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of S100 Calcium Binding Protein A6 (S100A6) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 15.6-1,000pg/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100 Calcium Binding Protein A6 (S100A6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100 Calcium Binding Protein A6 (S100A6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Serum, plasma, tissue homogenates and other biological fluids. | ||||||||||||||||||||||
Sensitivity | < 0.057 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of S100 Calcium Binding Protein A6 (S100A6). No significant cross-reactivity or interference between S100 Calcium Binding Protein A6 (S100A6) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
Gene Symbol | S100a6 |
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Gene Full Name | S100 calcium binding protein A6 (calcyclin) |
Alias Symbols | 2A;2A9;5B10;Cac;Cacy;CALC;calcium binding protein A6 (calcyclin);CALCYCLIN;PRA;prolactin receptor-associated protein;protein S100-A6;S100 calcium-binding protein A6. |
NCBI Gene Id | 20200 |
Protein Name | Protein S100-A6 |
Description of Target | May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes such as the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative (By similarity). |
Uniprot ID | P14069 |
Protein Accession # | NP_035443 |
Nucleotide Accession # | NM_011313.2 |
Protein Size (# AA) | 89 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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