Catalog No: OKCD01366
Size:96WELLS
Price: $750.00
SKU
OKCD01366
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S100A4 High Sensitivity ELISA Kit (Human) (OKCD01366)

Datasheets/ManualsPrintable datasheet for S100A4 High Sensitivity ELISA Kit (Human) (OKCD01366)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)86-102%90-99%81-105%85-94%
EDTA Plasma (n=5)78-93%91-101%93-105%88-101%
Heparin Plasma (n=5)78-101%98-105%80-98%89-96%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive S100 Calcium Binding Protein A4 (S100A4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive S100 Calcium Binding Protein A4 (S100A4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive S100 Calcium Binding Protein A4 (S100A4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive S100 Calcium Binding Protein A4 (S100A4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range78-5,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)80-9781
EDTA Plasma (n=5)79-10385
Heparin Plasma (n=5)78-9581
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive S100 Calcium Binding Protein A4 (S100A4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive S100 Calcium Binding Protein A4 (S100A4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-S100A4 Microplate96 Wells (12 x 8 Well Strips)
S100A4 Lyophilized Standard2
100X Biotinylated S100A4 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates and other biological fluids
Sensitivity30 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of S100 Calcium Binding Protein A4 (S100A4). No significant cross-reactivity or interference between S100 Calcium Binding Protein A4 (S100A4) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolS100A4
Gene Full NameS100 calcium binding protein A4
Alias Symbols18A2;42A;calcium placental protein;Calvasculin;CAPL;fibroblast-specific protein-1;FSP1;leukemia multidrug resistance associated protein;malignant transformation suppression 1;Metastasin;metastasin 1;MTS1;murine placental homolog;P9KA;PEL98;placental calcium-binding protein;protein Mts1;protein S100-A4;S100 calcium-binding protein A4;S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog).
NCBI Gene Id6275
Protein NameProtein S100-A4
Description of TargetProtein S100-A4
Uniprot IDP26447
Protein Accession #NP_002952.1
Nucleotide Accession #NM_002961.2
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