S-Adenosyl methionine ELISA Kit (OKEH02593)

Catalog No: OKEH02593

Size:96T

S-Adenosyl methionine ELISA Kit (OKEH02593) standard curve.

Product Info

• Predicted Species Reactivity: All Species
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology S-Adenosyl methionine ELISA Kit (OKEH02593) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-S-Adenosyl methionine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated S-Adenosyl methionine and incubated. The S-Adenosyl methionine found in the sample or standards competes with the biotinylated S-Adenosyl methionine for limited binding sites on the immobilized anti-S-Adenosyl methionine antibody. Excess unbound biotinylated S-Adenosyl methionine and sample or standard S-Adenosyl methionine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated S-Adenosyl methionine captured in the well and inversely proportional to the amount of S-Adenosyl methionine which was contained in the sample or standard.
• ELISA Kit Range: 1.56-100nmol/L
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.1% (n = 20)
  Mean Inter-assay CV%: < 7.4% (n = 20)
• ELISA Kit Component:
  • Anti-S-Adenosyl methionine Microplate: 96 Wells (12 x 8 Well strips)
  • S-Adenosyl methionine Lyophilized Standard: 2 x 100 nmol
  • Sample Diluent: 1 x 20 mL
  • 100X S-Adenosyl methionine-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Target Abbr: SAM
  Target Name: S-Adenosyl methionine
  Pubchem: 34756
  Chemical formula: C₁₅H₂₃N₆O₅S⁺
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
• Sensitivity: 0.38nmol/L
• Specificity: Natural and recombinant General S-Adenosyl methionine
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Alias Symbols: SAM, S-Adenosyl methionine, SAM
• Protein Name: S-Adenosyl methionine
• Description of Target: S-Adenosyl methionine (ademetionine, AdoMet, SAM, SAMe, SAM-e) is a common cosubstrate involved in methyl group transfers. SAM was first discovered in Italy by G. L. Cantoni in 1952. It is made from adenosine triphosphate (ATP) and methionine by methionine adenosyltransferase (EC 2.5.1.6). Transmethylation, transsulfuration, and aminopropylation are the metabolic pathways that use SAM. Although these anabolic reactions occur throughout the body, most SAM is produced and consumed in the liver. The methyl group (CH3) attached to the methionine sulfur atom in SAM is chemically reactive. This allows donation of this group to an acceptor substrate in transmethylation reactions. More than 40 metabolic reactions involve the transfer of a methyl group from SAM to various substrates, such as nucleic acids, proteins, lipids and secondary metabolites. In bacteria, SAM is bound by the SAM riboswitch, which regulates genes involved in methionine or cysteine biosynthesis.