RXRG ELISA Kit (Human) (OKCD02858)

Catalog No: OKCD02858

Size:96WELLS

Human RXRG ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Retinoid X Receptor Gamma (RXRg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Retinoid X Receptor Gamma (RXRg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Retinoid X Receptor Gamma (RXRg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Retinoid X Receptor Gamma (RXRg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78-5,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Retinoid X Receptor Gamma (RXRg) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Retinoid X Receptor Gamma (RXRg) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-RXRG Microplate: 96 Wells (12 x 8 Well strips)
  • RXRG Lyophilized Standard: 2
  • 100X Biotinylated RXRG Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids
• Sensitivity: 0.057 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Retinoid X Receptor Gamma (RXRg). No significant cross-reactivity or interference between Retinoid X Receptor Gamma (RXRg) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: RXRG
• Gene Full Name: retinoid X receptor gamma
• Alias Symbols: NR2B3;nuclear receptor subfamily 2 group B member 3;retinoic acid receptor RXR-gamma;Retinoid X receptor gamma;RXRC.
• NCBI Gene Id: 6258
• Protein Name: Retinoic acid receptor RXR-gamma
• Description of Target: Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid (By similarity).
• Uniprot ID: P48443
• Protein Accession #: NP_001243500.1
• Nucleotide Accession #: NM_001256571.1
• Protein Size (# AA): 463