RUNX2 Antibody - middle region (ARP36678_T100)

Catalog No: ARP36678_T100

Host: Rabbit
Target Name: RUNX2
Sample Tissue: Human Jurkat
Antibody Dilution: 1.0ug/ml

Host: Rabbit
Target: RUNX2
Positive control (+): Hela (HL)
Negative control (-): Human liver (LI)
Antibody concentration: 0.2ug/ml

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RUNX2
• Purification: Protein A purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Peptide Sequence: Synthetic peptide located within the following region: DTATSDFCLWPSTLSKKSQAGASELGPFSDPRQFPSISSLTESRFSNPRM
• Concentration: 1.0 mg/ml
• Blocking Peptide: For anti-RUNX2 (ARP36678_T100) antibody is Catalog # AAP36678 (Previous Catalog # AAPP08105)
• Reference: Guo,J., et al., (2006) Dev. Biol. 292 (1), 116-128
• Publications:

  Schuchardt, M. et al. Uridine adenosine tetraphosphate activation of the purinergic receptor P2Y enhances in vitro vascular calcification. Kidney Int. 81, 256-65 (2012). 21956191

Target Info

• Gene Symbol: RUNX2
• Gene Full Name: Runt-related transcription factor 2
• Alias Symbols: CCD, AML3, CCD1, CLCD, OSF2, CBFA1, OSF-2, PEA2aA, PEBP2aA, CBF-alpha-1
• NCBI Gene Id: 860
• Protein Name: Runt-related transcription factor 2
• Description of Target: RUNX2 is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis, acting as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants, encoding different protein isoforms, result from alternate promoter use as well as alternate splicing.
• Uniprot ID: Q13950
• Protein Accession #: NP_001019801
• Nucleotide Accession #: NM_001024630
• Protein Size (# AA): 521
• Molecular Weight: 57kDa
• Protein Interactions: HDAC7; HDAC6; TP53; UBC; RB1; PML; HIF1A; SMURF1; SMAD1; SMURF2; HIVEP3; WWP1; FHL2; UBTF; SUV39H1; HDAC1; STAT3; SMAD3; RBM14; SOX9; NR0B2; EP300; HDAC5; HDAC4; HDAC3; CREBBP; XRCC5; HES1; XRCC6; BMPR1A; KAT2B; TLE1; YAP1; KAT6B; TAF1A; AXIN1; SMAD6; MAP