Catalog No: OAAF00715 (Formerly GWB-ASC121)
Size:100 ug
Price: $344.00
SKU
OAAF00715
Availability: Domestic: within 1-2 week delivery | International: 1-2 weeks
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Datasheets/ManualsPrintable datasheet for RUNX1 Antibody (OAAF00715)
Product Info
Predicted Species ReactivityHuman|Mouse|Rat
Product FormatLiquid
ClonalityPolyclonal
HostRabbit
ApplicationEnzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
Reconstitution and Storage-20°C
ImmunogenThe antiserum was produced against synthesized peptide derived from human AML1.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
SpecificityAML1 (Ab-276) antibody detects endogenous levels of total AML1 protein.
FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
DilutionThe optimal working dilution should be determined by the end user.
Application InfoWB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:5000
ReferenceChaitali Banerjee, PNAS, May 1996; 93: 4968. Jennifer J. Westendorf, Mol. Cell. Biol., Jan 1998; 18: 322 - 333. Ari Melnick, Blood, Dec 2000; 96: 3939 - 3947.
Gene SymbolRUNX1
Gene Full NameRUNX family transcription factor 1
Alias Symbolsacute myeloid leukemia 1 protein;AML1;AML1-ETO fusion;AML1-ETO fusion protein;AML1-EVI-1;AML1-EVI-1 fusion protein;AMLCR1;CBF2alpha;CBFA2;Core-binding factor subunit alpha-2;core-binding factor, runt domain, alpha subunit 2;EVI-1;mutant RUNX1;oncogene AML-1;PEA2-alpha B;PEBP2aB;PEBP2alpha;PEBP2-alpha B;polyomavirus enhancer-binding protein 2 alpha B subunit;runt related transcription factor 1;runt-related transcription factor 1;SL3/AKV core-binding factor alpha B subunit;SL3-3 enhancer factor 1 alpha B subunit.
NCBI Gene Id861
Protein NameRunt-related transcription factor 1
Description of TargetForms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (Probable). Essential for the development of normal hematopoiesis (PubMed:17431401). Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the BLK promoter (PubMed:10207087, PubMed:14970218). Inhibits KAT6B-dependent transcriptional activation (By similarity). Involved in lineage commitment of immature T cell precursors. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing (By similarity). Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down-regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells (PubMed:17377532). Positively regulates the expression of RORC in T-helper 17 cells (By similarity).
Uniprot IDQ01196
Protein Accession #NP_001001890.1
Molecular Weight48 kDa
  1. What is the species homology for "RUNX1 Antibody (OAAF00715)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".

  2. How long will it take to receive "RUNX1 Antibody (OAAF00715)"?

    This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".

  3. What buffer format is "RUNX1 Antibody (OAAF00715)" provided in?

    This item is provided in "Liquid".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "RUNX1 Antibody (OAAF00715)"?

    This target may also be called "acute myeloid leukemia 1 protein;AML1;AML1-ETO fusion;AML1-ETO fusion protein;AML1-EVI-1;AML1-EVI-1 fusion protein;AMLCR1;CBF2alpha;CBFA2;Core-binding factor subunit alpha-2;core-binding factor, runt domain, alpha subunit 2;EVI-1;mutant RUNX1;oncogene AML-1;PEA2-alpha B;PEBP2aB;PEBP2alpha;PEBP2-alpha B;polyomavirus enhancer-binding protein 2 alpha B subunit;runt related transcription factor 1;runt-related transcription factor 1;SL3/AKV core-binding factor alpha B subunit;SL3-3 enhancer factor 1 alpha B subunit." in publications.

  5. What is the shipping cost for "RUNX1 Antibody (OAAF00715)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "RUNX1 Antibody (OAAF00715)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "RUNX1 Antibody (OAAF00715)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "48 kDa".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "RUNX1 Antibody (OAAF00715)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "RUNX1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "RUNX1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "RUNX1"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "RUNX1"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "RUNX1"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "RUNX1"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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