RRM2 ELISA Kit (Rat) : 96 Wells (OKEH02214)

Catalog No: OKEH02214

Size:96T

Rat RRM2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology RRM2 ELISA Kit (Rat) (OKEH02214) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Rat RRM2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated RRM2 and incubated. The RRM2 found in the sample or standards competes with the biotinylated RRM2 for limited binding sites on the immobilized anti-Rat RRM2 antibody. Excess unbound biotinylated RRM2 and sample or standard RRM2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated RRM2 captured in the well and inversely proportional to the amount of RRM2 which was contained in the sample or standard.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.1% (n = 20)
  Mean Inter-assay CV%: < 8.3% (n = 20)
• ELISA Kit Component:
  • Anti-RRM2 Microplate: 96 Wells (12 x 8 Well strips)
  • RRM2 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X SREBF2-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.156 ng/mL
• Specificity: Natural and recombinant Rat Ribonucleoside-diphosphate reductase subunit M2
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: Rrm2
• Gene Full Name: ribonucleotide reductase regulatory subunit M2
• Alias Symbols: ribonucleoside-diphosphate reductase subunit M2;Ribonucleotide reductase 2;ribonucleotide reductase M2 polypeptide;ribonucleotide reductase small chain;ribonucleotide reductase small subunit.
• NCBI Gene Id: 362720
• Protein Name: Q4KLN6
• Description of Target: Ribonucleoside-diphosphate reductase subunit M2
• Uniprot ID: https://www.uniprot.org/uniprot/Q4KLN6
• Protein Accession #: NP_001020911.1
• Nucleotide Accession #: NM_001025740.1
• Protein Size (# AA): 390
• Molecular Weight: 45 kDa