RRBP1 Antibody (OAGA00131)

Catalog No: OAGA00131

Size:100 ul

Ribosome binding protein 1 antibody validation by siRNA knock-down.
Upperpanel: Ribosome binding protein 1 antibody OAGA00131
Lower panel: Vinculin antibody
A. 30 ?g HepG2 whole cell lysate/extract
B. 30 ?g whole cell lysate/extract of RRBP1 siRNA#1-transfected HepG2 cells
C. 30 ?g whole cell lysate/extract of RRBP1 siRNA#2-transfected HepG2 cells
5 % SDS-PAGE
Ribosome binding protein 1 antibody (OAGA00131) dilution: 1:500
Vinculin antibody dilution: 1:1000

Sample (30 ug of whole cell lysate)
A:NIH-3T3
5% SDS PAGE
OAGA00131 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using Ribosome binding protein 1(OAGA00131) antibody at 1:500 dilution.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Ribosome binding protein 1(OAGA00131) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with(Red) at 1:500.

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Ribosome binding protein 1(OAGA00131) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with(Red) at 1:2500.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: Analysis of cDNA clones indicates that ribosome binding protein 1 may exist in different forms due to removal of tandem repeats, or partial intraexonic splicing of RRBP1. The form presented here is lacking the canine p180 ribosome-binding domain, NQGKKAEGAQ, which is tandemly repeated close to the N-terminus in other forms that haven't been fully characterized. RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq]
  Positive Controls: HepG2, NIH-3T3
  Cellular Localization: Endoplasmic reticulum membrane; Single-pass type III membrane protein
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1183 and 1410 of Ribosome binding protein 1 (Uniprot ID#Q9P2E9)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: RRBP1
• Gene Full Name: Ribosome-binding protein 1
• Alias Symbols: RRp, hES, p180, ES130, ES/130
• NCBI Gene Id: 6238
• Protein Name: Ribosome-binding protein 1
• Uniprot ID: Q9P2E9
• Protein Accession #: NP_001036041.1
• Molecular Weight: 109 kDa