RPS6KA3 Antibody (OAAF01558)

Catalog No: OAAF01558 (Formerly GWB-ASC920)

Size:100 ug

Western blot analysis of extracts from Jurkat cells, using RSK1/2/3/4 Antibody. The lane on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human RSK1/2/3/4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: RSK1/2/3/4 (Ab-221/227/218/232) antibody detects endogenous levels of total RSK1/2/3/4 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; ELISA: 1:20000
• Reference: Moller D.E., Am. J. Physiol. 266:C351-C359(1994).
  Gregory S.G., Nature 441:315-321(2006).
  The MGC Project Team; Genome Res. 14-2121-2127(2004).

Target Info

• Gene Symbol: RPS6KA1|RPS6KA2|RPS6KA3|RPS6KA6
• Gene Full Name: ribosomal protein S6 kinase A1|ribosomal protein S6 kinase A2|ribosomal protein S6 kinase A3|ribosomal protein S6 kinase A6
• Alias Symbols: 90 kDa ribosomal protein S6 kinase 1;90 kDa ribosomal protein S6 kinase 2;90 kDa ribosomal protein S6 kinase 3;90 kDa ribosomal protein S6 kinase 6;CLS;dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1);epididymis secretory sperm binding protein;HU-1;HU-2;HU-3;insulin-stimulated protein kinase 1;ISPK-1;MAP kinase-activated protein kinase 1a;MAP kinase-activated protein kinase 1b;MAP kinase-activated protein kinase 1c;MAPK-activated protein kinase 1a;MAPK-activated protein kinase 1b;MAPK-activated protein kinase 1c;MAPKAP kinase 1a;MAPKAP kinase 1b;MAPKAPK1;MAPKAPK-1a;MAPKAPK1A;MAPKAPK-1b;MAPKAPK1B;MAPKAPK1C;mitogen-activated protein kinase-activated protein kinase 1C;MRX19;p90Rsk;p90-RSK 1;p90-RSK 3;p90-RSK 6;p90RSK1;p90RSK2;p90-RSK2;p90-RSK3;p90RSK6;p90S6K;pp90RSK2;pp90RSK3;PP90RSK4;ribosomal protein S6 kinase alpha-1;ribosomal protein S6 kinase alpha-2;ribosomal protein S6 kinase alpha-3;ribosomal protein S6 kinase alpha-6;ribosomal protein S6 kinase, 90kDa, polypeptide 1;ribosomal protein S6 kinase, 90kDa, polypeptide 2;ribosomal protein S6 kinase, 90kDa, polypeptide 3;ribosomal protein S6 kinase, 90kDa, polypeptide 6;ribosomal S6 kinase 1;ribosomal S6 kinase 2;ribosomal S6 kinase 3;ribosomal S6 kinase 4;RSK;RSK1;RSK-1;RSK2;RSK-2;RSK3;RSK4;RSK-4;S6K-alpha;S6K-alpha 1;S6K-alpha 6;S6K-alpha2;S6K-alpha3;S6K-alpha-3;S6K-alpha-6;XLID19.
• NCBI Gene Id: 27330|6195|6196|6197
• Protein Name: Ribosomal protein S6 kinase alpha-1|Ribosomal protein S6 kinase alpha-2|Ribosomal protein S6 kinase alpha-3|Ribosomal protein S6 kinase alpha-6
• Description of Target: Constitutively active serine/threonine-protein kinase that exhibits growth-factor-independent kinase activity and that may participate in p53/TP53-dependent cell growth arrest signaling and play an inhibitory role during embryogenesis.|Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1 and histone H3 at 'Ser-10', which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the preinitiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression. In LPS-stimulated dendritic cells, is involved in TLR4-induced macropinocytosis, and in myeloma cells, acts as effector of FGFR3-mediated transformation signaling, after direct phosphorylation at Tyr-529 by FGFR3. Negatively regulates EGF-induced MAPK1/3 phosphorylation via phosphorylation of SOS1. Phosphorylates SOS1 at 'Ser-1134' and 'Ser-1161' that create YWHAB and YWHAE binding sites and which contribute to the negative regulation of MAPK1/3 phosphorylation (By similarity). Phosphorylates EPHA2 at 'Ser-897', the RPS6KA-EPHA2 signaling pathway controls cell migration (PubMed:26158630).|Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the pre-initiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro-apoptotic proteins BAD and DAPK1 and suppressing their pro-apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Mediates induction of hepatocyte prolifration by TGFA through phosphorylation of CEBPB (By similarity). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression. Phosphorylates EPHA2 at 'Ser-897', the RPS6KA-EPHA2 signaling pathway controls cell migration (PubMed:26158630).|Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of transcription factors, regulates translation, and mediates cellular proliferation, survival, and differentiation. May function as tumor suppressor in epithelial ovarian cancer cells.
• Uniprot ID: P51812|Q15349|Q15418|Q9UK32
• Protein Accession #: NP_055311.1
• Molecular Weight: 82 kDa