RPS3A Antibody (OAGA00372)

Catalog No: OAGA00372

Size:100 ul

Sample(30 ug whole cell lysate)
A: 293T
B: A431
C: H1299
D: HeLa S3
E: Hep G2
F: MOLT4
G: Raji
12% SDS PAGE
OAGA00372 diluted at 1:1000

RPS3A antibody detects RPS3A protein by Western blot analysis.
A. 30 ug Neuro2A whole cell lysate/extract
B. 30 ug GL261 whole cell lysate/extract
C. 30 ug C8D30 whole cell lysate/extract
D. 30 ug NIH-3T3 whole cell lysate/extract
E. 30 ug BCL-1 whole cell lysate/extract
F. 30 ug Raw264.7 whole cell lysate/extract
G. 30 ug C2C12 whole cell lysate/extract
12 % SDS-PAGE
RPS3A antibody (OAGA00372) dilution: 1:1000

RPS3A antibody detects RPS3A protein by Western blot analysis.
A. 30 ug PC-12 whole cell lysate/extract
B. 30 ug Rat2 whole cell lysate/extract
12 % SDS-PAGE
RPS3A antibody (OAGA00372) dilution: 1:1000

RPS3A antibody detects RPS3A protein at cytosol on mouse brain stem by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain stem.
RPS3A antibody (OAGA00372) dilution: 1:200.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ribosomal protein S3a(OAGA00372) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S3AE family of ribosomal proteins. It is located in the cytoplasm. Disruption of the gene encoding rat ribosomal protein S3a, also named v-fos transformation effector protein, in v-fos-transformed rat cells results in reversion of the transformed phenotype. Transcript variants utilizing alternative transcription start sites have been described. This gene is co-transcribed with the U73A and U73B small nucleolar RNA genes, which are located in its fourth and third introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]
  Positive Controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, Neuro 2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, PC-12, Rat2
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 14 and 264 of RPS3A (Uniprot ID#P61247)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: RPS3A
• Gene Full Name: 40S ribosomal protein S3a
• Alias Symbols: S3A, FTE1, MFTL
• NCBI Gene Id: 6189
• Protein Name: 40S ribosomal protein S3a
• Uniprot ID: P61247
• Protein Accession #: NP_000997.1
• Molecular Weight: 30 kDa