RPS27A Antibody (OAGA01580)

Catalog No: OAGA01580

Size:100 ul

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using RPS27A (OAGA01580) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled (Red) at 1:2000.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IF, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human RPS27A. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.4mg/ml
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Target Info

• Gene Symbol: RPS27A
• Gene Full Name: ribosomal protein S27a
• Alias Symbols: UBC, S27A, CEP80, UBA80, HEL112, UBCEP1, UBCEP80
• NCBI Gene Id: 6233
• Description of Target: Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.
• Uniprot ID: P62979